Miguel A. R. B. Castanho
Miguel A. R. B. Castanho graduated in biochemistry, University of Lisbon, in 1990, and received his Ph.D. degree in 1993, Technical University of Lisbon. The Habilitation degree was awarded in 1999, University of Lisbon, for his research and teaching work in the field of molecular biophysics. M. Castanho uses optical spectroscopies such fluorescence emission, light scattering, and UV-Vis absorption to unravel structural details of lipid membrane-drug interactions. His general methodologies have been derived to estimate the depth of insertion and orientation of drugs in lipid bilayers, for instance. Since 2000, M. Castanho’s lab has focused particularly on peptide drugs: analgesic, antimicrobial, cell-penetranting or viral fusion inhibitors. For the work of the team he supervises, M. Castanho has received two Dr. Champalimaud awards (Ministry of health, Portugal) and the Vicente de Seabra medal (Portuguese Chemical Society). M. Castanho is a Full Professor in biochemistry, Faculty of Medicine, University of Lisbon, and Member of the Board of direction of the Portuguese Biophysical Society.
Biography Updated on 21 January 2008
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Articles in Scholarly Journals [Incomplete List]
- Shiga toxin B-subunit sequential binding to its natural receptor in lipid membranes
Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1768, no. 3, pp. 628–636, 2007 - Omiganan interaction with bacterial membranes and cell wall models. Assigning a biological role to saturation
Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1768, no. 5, pp. 1277–1290, 2007 - How to address CPP and AMP translocation? Methods to detect and quantify peptide internalization in vitro and in vivo (Review)
Molecular Membrane Biology, vol. 24, no. 3, pp. 173–184, 2007 - Energy-independent translocation of cell-penetrating peptides occurs without formation of pores. A biophysical study with pep-1
Molecular Membrane Biology, vol. 24, no. 4, pp. 282–293, 2007 - The influence of cholesterol on the interaction of HIV gp41 membrane proximal region-derived peptides with lipid bilayers
FEBS Journal, vol. 274, no. 19, pp. 5096–5104, 2007 - The Journal of Physical Chemistry B, vol. 110, no. 7, pp. 3385–3394, 2006
- Cell-penetrating peptides and antimicrobial peptides: how different are they?
Biochemical Journal, vol. 399, no. 1, p. 1, 2006 - Chiral Recognition ofD-Kyotorphin by Lipidic Membranes: Relevance Toward Improved Analgesic Efficiency
ChemMedChem, vol. 1, no. 7, pp. 723–728, 2006 - The membranes’ role in the HIV-1 neutralizing monoclonal antibody 2F5 mode of action needs re-evaluation
Antiviral Research, vol. 71, no. 1, pp. 69–72, 2006 - Why are HIV-1 fusion inhibitors not effective against SARS-CoV? Biophysical evaluation of molecular interactions
Biochimica et Biophysica Acta (BBA) - General Subjects, vol. 1760, no. 1, pp. 55–61, 2006 - Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1669, no. 2, pp. 75–86, 2005
- Lipidic Membranes Are Potential ?Catalysts? in the Ligand Activity of the Multifunctional Pentapeptide Neokyotorphin
ChemBioChem, vol. 6, no. 4, pp. 697–702, 2005 - Re-evaluating the role of strongly charged sequences in amphipathic cell-penetrating peptidesA fluorescence study using Pep-1
FEBS Letters, vol. 579, no. 20, pp. 4498–4502, 2005 - Lipid membrane-induced optimization for ligand–receptor docking: recent tools and insights for the “membrane catalysis” model
European Biophysics Journal, vol. 35, no. 2, pp. 92–103, 2005 - Miguel A.R.B. Castanho
European Biophysics Journal, vol. 35, no. 2, pp. 91–91, 2005 - Developing students' awareness of public understanding of science: A matter of survival?
Biochemistry and Molecular Biology Education, vol. 33, no. 3, pp. 202–204, 2005 - Biochemistry, vol. 44, no. 30, pp. 10189–10198, 2005
- Overview of Common Spectroscopic Methods to Determine the Orientation/Alignment of Membrane Probes and Drugs in Lipidic Bilayers
Current Organic Chemistry, vol. 9, no. 9, pp. 889–898, 2005 - Does Aliphatic Chain Length Influence Carbocyanines' Orientation in Supported Lipid Multilayers?
Journal of Fluorescence, vol. 14, no. 3, pp. 281–287, 2004 - Putative role of membranes in the HIV fusion inhibitor enfuvirtide mode of action at the molecular level
Biochemical Journal, vol. 377, no. 1, p. 107, 2004 - Journal of the American Chemical Society, vol. 126, no. 45, pp. 14758–14763, 2004
- Biochemistry, vol. 43, no. 30, pp. 9716–9724, 2004
- An overview of the biophysical applications of atomic force microscopy
Biophysical Chemistry, vol. 107, no. 2, pp. 133–149, 2004 - Cholesterol modulates maculosin's orientation in model systems of biological membranesRelevance towards putative molecular recognition
Steroids, vol. 69, no. 13-14, pp. 825–830, 2004 - Evaluation of Lipopolysaccharide Aggregation by Light Scattering Spectroscopy
ChemBioChem, vol. 4, no. 1, pp. 96–100, 2003 - Quantifying molecular partition into model systems of biomembranes: an emphasis on optical spectroscopic methods
Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1612, no. 2, pp. 123–135, 2003 - Brownian dynamics simulation of the unsaturated lipidic molecules oleic and docosahexaenoic acid confined in a cellular membrane
Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1565, no. 1, pp. 29–35, 2002 - Joint determination by Brownian dynamics and fluorescence quenching of the in-depth location profile of biomolecules in membranes
Analytical Biochemistry, vol. 307, no. 1, pp. 1–12, 2002 - Dealing with the challenges of teaching molecular biophysics to biochemistry majors through an heuristics-based approach
Biochemistry and Molecular Biology Education, vol. 30, no. 3, pp. 163–168, 2002 - The Journal of Physical Chemistry B, vol. 106, no. 29, pp. 7278–7282, 2002
- The Journal of Physical Chemistry B, vol. 105, no. 2, pp. 562–568, 2001
- A photophysical study of the polyene antibiotic filipin Self-aggregation and filipin–ergosterol interaction
Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1510, no. 1-2, pp. 125–135, 2001 - Crude tall-oil sodium salts micellization in aqueous solutions studied by static and dynamic light scattering
Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 191, no. 3, pp. 263–268, 2001 - The Journal of Physical Chemistry B, vol. 104, no. 48, pp. 11579–11584, 2000
- Conformation and dynamic properties of a saturated hydrocarbon chain confined in a model membrane: a Brownian dynamics simulation
Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1463, no. 1, pp. 131–141, 2000 - The pentaene macrolide antibiotic filipin prefers more rigid DPPC bilayers: a fluorescence pressure dependence study
Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1419, no. 1, pp. 1–14, 1999 - Simulation of the distribution and diffusion of a rigid amphipathic particle embedded in a model membrane
Biophysical Chemistry, vol. 79, no. 1, pp. 41–53, 1999 - Continuous particle size distribution analysis with dynamic light scattering MAXAMPER: A regularization method using the maximum amplitude for the average error and the Lagrange's multipliers method
Journal of Biochemical and Biophysical Methods, vol. 36, no. 2-3, pp. 101–117, 1998 - Biochemistry, vol. 37, no. 24, pp. 8674–8682, 1998
- Fluorescence quenching data interpretation in biological systems The use of microscopic models for data analysis and interpretation of complex systems
Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1373, no. 1, pp. 1–16, 1998 - Structural characterization of organized systems of polysaccharides and phospholipids by light scattering spectroscopy and electron microscopy
Carbohydrate Research, vol. 300, no. 1, pp. 31–40, 1997 - Structural characterization (shape and dimensions) and stability of polysaccharide/lipid nanoparticles
Biopolymers, vol. 41, no. 5, pp. 511–520, 1997 - Separating the turbidity spectra of vesicles from the absorption spectra of membrane probes and other chromophores
European Biophysics Journal, vol. 26, no. 3, pp. 253–259, 1997 - The transverse location of the fluorescent probe trans-parinaric acid in lipid bilayers
Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1279, no. 2, pp. 164–168, 1996 - Fluorescence study of a derivatized diacylglycerol incorporated in model membranes
Chemistry and Physics of Lipids, vol. 69, no. 1, pp. 75–85, 1994 - Filipin and its interaction with cholesterol in aqueous media studied using static and dynamic light scattering
Biopolymers, vol. 34, no. 4, pp. 447–456, 1994 - Fluorescence study of the macrolide pentaene antibiotic filipin in aqueous solution and in a model system of membranes
European Journal of Biochemistry, vol. 207, no. 1, pp. 125–134, 1992