Miguel A. R. B. Castanho

Miguel A. R. B. Castanho graduated in biochemistry, University of Lisbon, in 1990, and received his Ph.D. degree in 1993, Technical University of Lisbon. The Habilitation degree was awarded in 1999, University of Lisbon, for his research and teaching work in the field of molecular biophysics. M. Castanho uses optical spectroscopies such fluorescence emission, light scattering, and UV-Vis absorption to unravel structural details of lipid membrane-drug interactions. His general methodologies have been derived to estimate the depth of insertion and orientation of drugs in lipid bilayers, for instance. Since 2000, M. Castanho’s lab has focused particularly on peptide drugs: analgesic, antimicrobial, cell-penetranting or viral fusion inhibitors. For the work of the team he supervises, M. Castanho has received two Dr. Champalimaud awards (Ministry of health, Portugal) and the Vicente de Seabra medal (Portuguese Chemical Society). M. Castanho is a Full Professor in biochemistry, Faculty of Medicine, University of Lisbon, and Member of the Board of direction of the Portuguese Biophysical Society.

Biography Updated on 21 January 2008

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Articles in Scholarly Journals [Incomplete List]

  1. Shiga toxin B-subunit sequential binding to its natural receptor in lipid membranes
    Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1768, no. 3, pp. 628–636, 2007
  2. Omiganan interaction with bacterial membranes and cell wall models. Assigning a biological role to saturation
    Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1768, no. 5, pp. 1277–1290, 2007
  3. How to address CPP and AMP translocation? Methods to detect and quantify peptide internalization in vitro and in vivo (Review)
    Molecular Membrane Biology, vol. 24, no. 3, pp. 173–184, 2007
  4. Energy-independent translocation of cell-penetrating peptides occurs without formation of pores. A biophysical study with pep-1
    Molecular Membrane Biology, vol. 24, no. 4, pp. 282–293, 2007
  5. The influence of cholesterol on the interaction of HIV gp41 membrane proximal region-derived peptides with lipid bilayers
    FEBS Journal, vol. 274, no. 19, pp. 5096–5104, 2007
  6. The Journal of Physical Chemistry B, vol. 110, no. 7, pp. 3385–3394, 2006
  7. Cell-penetrating peptides and antimicrobial peptides: how different are they?
    Biochemical Journal, vol. 399, no. 1, p. 1, 2006
  8. Chiral Recognition ofD-Kyotorphin by Lipidic Membranes: Relevance Toward Improved Analgesic Efficiency
    ChemMedChem, vol. 1, no. 7, pp. 723–728, 2006
  9. The membranes’ role in the HIV-1 neutralizing monoclonal antibody 2F5 mode of action needs re-evaluation
    Antiviral Research, vol. 71, no. 1, pp. 69–72, 2006
  10. Why are HIV-1 fusion inhibitors not effective against SARS-CoV? Biophysical evaluation of molecular interactions
    Biochimica et Biophysica Acta (BBA) - General Subjects, vol. 1760, no. 1, pp. 55–61, 2006
  11. Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1669, no. 2, pp. 75–86, 2005
  12. Lipidic Membranes Are Potential ?Catalysts? in the Ligand Activity of the Multifunctional Pentapeptide Neokyotorphin
    ChemBioChem, vol. 6, no. 4, pp. 697–702, 2005
  13. Re-evaluating the role of strongly charged sequences in amphipathic cell-penetrating peptidesA fluorescence study using Pep-1
    FEBS Letters, vol. 579, no. 20, pp. 4498–4502, 2005
  14. Lipid membrane-induced optimization for ligand–receptor docking: recent tools and insights for the “membrane catalysis” model
    European Biophysics Journal, vol. 35, no. 2, pp. 92–103, 2005
  15. Miguel A.R.B. Castanho
    European Biophysics Journal, vol. 35, no. 2, pp. 91–91, 2005
  16. Developing students' awareness of public understanding of science: A matter of survival?
    Biochemistry and Molecular Biology Education, vol. 33, no. 3, pp. 202–204, 2005
  17. Biochemistry, vol. 44, no. 30, pp. 10189–10198, 2005
  18. Overview of Common Spectroscopic Methods to Determine the Orientation/Alignment of Membrane Probes and Drugs in Lipidic Bilayers
    Current Organic Chemistry, vol. 9, no. 9, pp. 889–898, 2005
  19. Does Aliphatic Chain Length Influence Carbocyanines' Orientation in Supported Lipid Multilayers?
    Journal of Fluorescence, vol. 14, no. 3, pp. 281–287, 2004
  20. Putative role of membranes in the HIV fusion inhibitor enfuvirtide mode of action at the molecular level
    Biochemical Journal, vol. 377, no. 1, p. 107, 2004
  21. Journal of the American Chemical Society, vol. 126, no. 45, pp. 14758–14763, 2004
  22. Biochemistry, vol. 43, no. 30, pp. 9716–9724, 2004
  23. An overview of the biophysical applications of atomic force microscopy
    Biophysical Chemistry, vol. 107, no. 2, pp. 133–149, 2004
  24. Cholesterol modulates maculosin's orientation in model systems of biological membranesRelevance towards putative molecular recognition
    Steroids, vol. 69, no. 13-14, pp. 825–830, 2004
  25. Evaluation of Lipopolysaccharide Aggregation by Light Scattering Spectroscopy
    ChemBioChem, vol. 4, no. 1, pp. 96–100, 2003
  26. Quantifying molecular partition into model systems of biomembranes: an emphasis on optical spectroscopic methods
    Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1612, no. 2, pp. 123–135, 2003
  27. Brownian dynamics simulation of the unsaturated lipidic molecules oleic and docosahexaenoic acid confined in a cellular membrane
    Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1565, no. 1, pp. 29–35, 2002
  28. Joint determination by Brownian dynamics and fluorescence quenching of the in-depth location profile of biomolecules in membranes
    Analytical Biochemistry, vol. 307, no. 1, pp. 1–12, 2002
  29. Dealing with the challenges of teaching molecular biophysics to biochemistry majors through an heuristics-based approach
    Biochemistry and Molecular Biology Education, vol. 30, no. 3, pp. 163–168, 2002
  30. The Journal of Physical Chemistry B, vol. 106, no. 29, pp. 7278–7282, 2002
  31. The Journal of Physical Chemistry B, vol. 105, no. 2, pp. 562–568, 2001
  32. A photophysical study of the polyene antibiotic filipin Self-aggregation and filipin–ergosterol interaction
    Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1510, no. 1-2, pp. 125–135, 2001
  33. Crude tall-oil sodium salts micellization in aqueous solutions studied by static and dynamic light scattering
    Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 191, no. 3, pp. 263–268, 2001
  34. The Journal of Physical Chemistry B, vol. 104, no. 48, pp. 11579–11584, 2000
  35. Conformation and dynamic properties of a saturated hydrocarbon chain confined in a model membrane: a Brownian dynamics simulation
    Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1463, no. 1, pp. 131–141, 2000
  36. The pentaene macrolide antibiotic filipin prefers more rigid DPPC bilayers: a fluorescence pressure dependence study
    Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1419, no. 1, pp. 1–14, 1999
  37. Simulation of the distribution and diffusion of a rigid amphipathic particle embedded in a model membrane
    Biophysical Chemistry, vol. 79, no. 1, pp. 41–53, 1999
  38. Continuous particle size distribution analysis with dynamic light scattering MAXAMPER: A regularization method using the maximum amplitude for the average error and the Lagrange's multipliers method
    Journal of Biochemical and Biophysical Methods, vol. 36, no. 2-3, pp. 101–117, 1998
  39. Biochemistry, vol. 37, no. 24, pp. 8674–8682, 1998
  40. Fluorescence quenching data interpretation in biological systems The use of microscopic models for data analysis and interpretation of complex systems
    Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1373, no. 1, pp. 1–16, 1998
  41. Structural characterization of organized systems of polysaccharides and phospholipids by light scattering spectroscopy and electron microscopy
    Carbohydrate Research, vol. 300, no. 1, pp. 31–40, 1997
  42. Structural characterization (shape and dimensions) and stability of polysaccharide/lipid nanoparticles
    Biopolymers, vol. 41, no. 5, pp. 511–520, 1997
  43. Separating the turbidity spectra of vesicles from the absorption spectra of membrane probes and other chromophores
    European Biophysics Journal, vol. 26, no. 3, pp. 253–259, 1997
  44. The transverse location of the fluorescent probe trans-parinaric acid in lipid bilayers
    Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1279, no. 2, pp. 164–168, 1996
  45. Fluorescence study of a derivatized diacylglycerol incorporated in model membranes
    Chemistry and Physics of Lipids, vol. 69, no. 1, pp. 75–85, 1994
  46. Filipin and its interaction with cholesterol in aqueous media studied using static and dynamic light scattering
    Biopolymers, vol. 34, no. 4, pp. 447–456, 1994
  47. Fluorescence study of the macrolide pentaene antibiotic filipin in aqueous solution and in a model system of membranes
    European Journal of Biochemistry, vol. 207, no. 1, pp. 125–134, 1992