After a Ph.D. degree in theoretical and physical chemistry, Bernard Gilquin joined, for 9 years, a computer services company as a group leader in computer-aided molecular softwares. Then Gilquin turned to protein science. Gilquin started as mainly involved in protein structure determination from NMR data. Then Gilquin was the Head of the Structural Biology and Radiobiology Laboratory, CEA Saclay. This laboratory belongs to the Institute of Biology and Technology of Saclay. Gilquin is now Scientific Consultant at iBiTec-S, France. Gilquin is mainly interested in the characterization of molecular assembly structures derived from NMR, EM, SAXS, and mutagenesis data. Coupling different biophysical methods for obtaining structural information at several resolutions on the same assembly seems to be currently one of the most challenging approaches in structural biology. The approach consists mainly in combining high-resolution data (from X-ray crystallography and NMR) with low-resolution data (from SAXS and EM) and with other heterogeneous information (binding data on mutants measured by different techniques). For example, Gilquin characterized the structure of bacteriophage SPP1 head-to-tail connector, gating mechanism for DNA ejection, and tail interaction in combining NMR and EM data. The combination of NMR and SAXS is particularly powerful to access three-dimensional information on partially folded structures such as that of MAN1, an inner nuclear membrane protein interacting with the transcription regulators SMAD2 and SMAD3. Structural modeling of the peptide ligands-GPCR complexes can be performed in combining structure of ligand and homology model of GPCR with several distance restraints derived from cycle-mutant experiments.
Biography Updated on 31 January 2013