David Kaplan received both M.D. and Ph.D. degrees from the University of Chicago, Ill, USA, and was a resident and a Postdoctoral Fellow at Washington University in Saint Louis. He has been on the faculty at Case Western Reserve University for over 25 years. His research has involved the development of human T cell clones, gene transfer into T cells, immune responses to viruses, and most recently flow cytometric technology. In 2008, he founded a biotechnology company, Pathfinder Biotech, to promulgate the use of his flow cytometric methods. He has developed an enzymatic amplification procedure for flow cytometry that increases the specific fluorescence signal between 10- and 100-fold thereby producing a significant enhancement in the resolving power of the technique. Using this technique, he has been able to detect the presence of molecules that could not be observed by the standard procedure. With enzymatic amplification staining, he has significantly enhanced the sensitivity of flow cytometric analysis for surface receptors and a variety of intracellular analytes such as cyclin molecules, phosphoantigens, transcription factors, mitochondrial molecules, apoptosis mediators, and RNA. Enzymatic amplification staining gives a resolving capability that is comparable to the sensitivity of western analysis.
Biography Updated on 29 March 2010