Toshio Yanagida

Toshio Yanagida received the Ph.D. degree from Osaka University, Japan, in 1976. He was an Associate Researcher, an Assistant Professor, and a Professor in the faculty of engineering science at the Osaka university, Japan, in 1974, 1987, and 1988, respectively. He led Biomotron Project, ERATO, JST, in 1992, Single molecule processes Project, ICORP, JST, in 1998 and Creation and Application of Soft Nano-Machine, the Hyperfunctional Molecular Machine Project, CREST, JST, in 2002. He was a Professor at the Osaka University Graduate School of Medicine in 1996. From 2002 to 2004, he was a Dean and Professor at the Osaka University Graduate School of Frontier Biosciences. Currently, he is a Professor at the Osaka University Graduate School of Frontier Biosciences. Professor Yanagida received 7th Osaka science award in 1989, 4th Tsukahara memotial award in 1992, 25th Naito memorial science promotion award in 1994, the Japan academy award and the imperial award in 1998, and 1998 Asahi award in 1999 for his outstanding achievement in the field of molecular motors and single molecule detection. Professor Yanagida is a Member of Japan biophysical society and USA biophysical society. He served as a President of Japan Biophysical Society and a Member of editorial board of Biophysical journal. He is a Counselor in USA Biophysical Society.

Biography Updated on 25 January 2008

Personal Home Page

http://www.jst.go.jp/erato/project/ysu_P/ysu_P.html

Articles in Scholarly Journals [Incomplete List]

  1. The diffusive search mechanism of processive myosin class-V motor involves directional steps along actin subunits
    Biochemical and Biophysical Research Communications, vol. 354, no. 2, pp. 379–384, 2007
  2. Cooperative actions between myosin heads bring effective functions
    Biosystems, vol. 88, no. 3, pp. 293–300, 2007
  3. Input–output relationship in galvanotactic response of Dictyostelium cells
    Biosystems, vol. 88, no. 3, pp. 261–272, 2007
  4. Stochastic signal inputs for chemotactic response in Dictyostelium cells revealed by single molecule imaging techniques
    Biosystems, vol. 88, no. 3, pp. 251–260, 2007
  5. Dynamic polymorphism of actin as activation mechanism for cell motility
    Biosystems, vol. 88, no. 3, pp. 273–282, 2007
  6. Brownian motion, fluctuation and life
    Biosystems, vol. 88, no. 3, pp. 228–242, 2007
  7. Single molecule thermodynamics in biological motors
    Biosystems, vol. 88, no. 3, pp. 283–292, 2007
  8. Single molecule FRET for the study on structural dynamics of biomolecules
    Biosystems, vol. 88, no. 3, pp. 243–250, 2007
  9. Role of Multiple Bonds Between the Single Cell Adhesion Molecules, Nectin and Cadherin, Revealed by High Sensitive Force Measurements
    Journal of Molecular Biology, vol. 367, no. 4, pp. 996–1006, 2007
  10. VEPs elicited by local correlations and global symmetry: Characteristics and interactions
    Vision Research, vol. 47, no. 16, pp. 2212–2222, 2007
  11. Myosin-V makes two brownian 90° rotations per 36-nm step
    Nature Structural & Molecular Biology, vol. 14, no. 10, Article ID nsmb1298, 5 pages, 2007
  12. Ischemic Vasoconstriction And Tissue Energy Metabolism during Global Cerebral Ischemia in Gerbils
    Journal of Neurotrauma, vol. 24, no. 3, pp. 547–558, 2007
  13. Immobilizing BK-channels in artificial lipid bilayers using annexin V
    e-Journal of Surface Science and Nanotechnology, vol. 5, pp. 1–5, 2007
  14. Telencephalin Slows Spine Maturation
    Journal of Neuroscience, vol. 26, no. 6, pp. 1776–1786, 2006
  15. Single-molecule analysis of chemoattractant-stimulated membrane recruitment of a PH-domain-containing protein
    Journal of Cell Science, vol. 119, no. 6, pp. 1071–1079, 2006
  16. Synthesis of Long-template DNA Using Enzymatic Reaction for Regular Alignment of Au-nanoparticles
    Chemistry Letters, vol. 35, no. 11, pp. 1290–1291, 2006
  17. Noncontact backscatter-mode near-infrared time-resolved imaging system: preliminary study for functional brain mapping
    Journal of Biomedical Optics, vol. 11, no. 5, p. 054006, 2006
  18. Single Supramolecule Spectroscopy of Natural and Alkaline-Treated Chlorosomes from Green Sulfur Photosynthetic Bacteria
    Journal of Nanoscience and Nanotechnology, vol. 6, no. 6, pp. 1750–1757, 2006
  19. Cargo-Binding Makes a Wild-Type Single-Headed Myosin-VI Move Processively
    Biophysical Journal, vol. 90, no. 10, pp. 3643–3652, 2006
  20. Single-molecule analysis of epidermal growth factor binding on the surface of living cells
    The EMBO Journal, vol. 25, no. 18, Article ID 7601308, 7 pages, 2006
  21. Epidermal growth factor-regulated activation of Rac GTPase enhances CD44 cleavage by metalloproteinase disintegrin ADAM10
    Biochemical Journal, vol. 395, no. 1, p. 65, 2006
  22. Dynamic polymorphism of single actin molecules in the actin filament
    Nature Chemical Biology, vol. 2, no. 2, Article ID nchembio763, 3 pages, 2006
  23. Tumor suppressor PTEN acts through dynamic interaction with the plasma membrane
    Proceedings of the National Academy of Sciences, vol. 103, no. 10, pp. 3633–3638, 2006
  24. Optical coherence tomography reveals in vivo cortical plasticity of adult mice in response to peripheral neuropathic pain
    Neuroscience Letters, vol. 397, no. 1-2, pp. 35–39, 2006
  25. Langmuir, vol. 22, no. 14, pp. 6302–6307, 2006
  26. Covalent immobilization of epidermal growth factor molecules for single-molecule imaging analysis of intracellular signaling
    Biomaterials, vol. 27, no. 18, pp. 3343–3350, 2006
  27. Formation of signal transduction complexes during immobile phase of NGFR movements
    Biochemical and Biophysical Research Communications, vol. 342, no. 1, pp. 316–322, 2006
  28. Dynamic polymorphism of Ras observed by single molecule FRET is the basis for molecular recognition
    Biochemical and Biophysical Research Communications, 2006
  29. A unique mechanism for the processive movement of single-headed myosin-IX
    Biochemical and Biophysical Research Communications, vol. 343, no. 4, pp. 1159–1164, 2006
  30. Lysenin forms a voltage-dependent channel in artificial lipid bilayer membranes
    Biochemical and Biophysical Research Communications, vol. 346, no. 1, pp. 288–292, 2006
  31. On Myosin II dynamics in the presence of external loads
    Biosystems, vol. 81, no. 2, pp. 163–175, 2005
  32. The sensorimotor transformation of cross-modal spatial information in the anterior intraparietal sulcus as revealed by functional MRI
    Cognitive Brain Research, vol. 22, no. 3, pp. 385–396, 2005
  33. The Neck Domain of Myosin II Primarily Regulates the Actomyosin Kinetics, not the Stepsize
    Journal of Molecular Biology, vol. 353, no. 2, pp. 213–221, 2005
  34. Entropy rectifies the Brownian steps of kinesin
    Nature Chemical Biology, vol. 1, no. 6, Article ID nchembio741, 5 pages, 2005
  35. Transcranial Ultrasonography System for Visualizing Skull and Brain Surface Aided by Fuzzy Expert System
    IEEE Transactions on Systems, Man and Cybernetics, Part B (Cybernetics), vol. 35, no. 6, pp. 1360–1373, 2005
  36. Dynamic Medicine, vol. 4, no. 1, p. 5, 2005
  37. Non-contact surface force microscopy for molecular interaction study
    e-Journal of Surface Science and Nanotechnology, vol. 3, pp. 46–50, 2005
  38. Single-Molecule Analysis of Epidermal Growth Factor Signaling that Leads to Ultrasensitive Calcium Response
    Biophysical Journal, vol. 88, no. 5, pp. 3720–3730, 2005
  39. Trafficking of a Ligand-Receptor Complex on the Growth Cones as an Essential Step for the Uptake of Nerve Growth Factor at the Distal End of the Axon: A Single-Molecule Analysis
    Journal of Neuroscience, vol. 25, no. 9, pp. 2181–2191, 2005
  40. Unconstrained Steps of Myosin VI Appear Longest among Known Molecular Motors
    Biophysical Journal, vol. 86, no. 6, pp. 3804–3810, 2004
  41. Single-Molecule Imaging of the Dynamic Interactions between Macromolecules
    Journal of Nanoscience and Nanotechnology, vol. 4, no. 6, pp. 616–621, 2004
  42. Changes in regional cerebral blood volume in frontal cortex during mental work with and without caffeine intake: functional monitoring using near-infrared spectroscopy
    Journal of Biomedical Optics, vol. 9, no. 4, p. 788, 2004
  43. Absorber's Effect Projected Directly Above Improves Spatial Resolution in Near Infrared Backscattered Imaging
    The Japanese Journal of Physiology, vol. 54, no. 1, pp. 79–86, 2004
  44. Mechano-chemical Coupling of Molecular Motors Revealed by Single Molecule Measurements
    Current Protein and Peptide Science, vol. 5, no. 2, pp. 81–87, 2004
  45. A one-headed class V myosin molecule develops multiple large ( 32-nm) steps successively
    Proceedings of the National Academy of Sciences, vol. 101, no. 26, pp. 9630–9635, 2004
  46. Circulatory basis of fMRI signals: relationship between changes in the hemodynamic parameters and BOLD signal intensity
    NeuroImage, vol. 21, no. 4, pp. 1204–1214, 2004
  47. EGF signalling amplification induced by dynamic clustering of EGFR
    Biochemical and Biophysical Research Communications, vol. 324, no. 3, pp. 1143–1149, 2004
  48. Monitoring Molecular Beacon DNA Probe Hybridization at the Single-Molecule Level
    Chemistry - A European Journal, vol. 9, no. 22, pp. 5686–5692, 2003
  49. Single- and Multiple-Molecule Dynamics of the Signaling from H-Ras to cRaf-1 Visualized on the Plasma Membrane of Living Cells
    ChemPhysChem, vol. 4, no. 7, pp. 748–753, 2003
  50. Noncontact Surface Force Microscopy of Protein Molecules
    ChemPhysChem, vol. 4, no. 12, pp. 1361–1364, 2003
  51. Stochastic properties of actomyosin motor
    Biosystems, vol. 71, no. 1-2, pp. 101–110, 2003
  52. Stochastic processes in nano-biomachines revealed by single molecule detection
    Biosystems, vol. 71, no. 1-2, pp. 233–244, 2003
  53. Single molecule processes on the stepwise movement of ATP-driven molecular motors
    Biosystems, vol. 71, no. 1-2, pp. 145–156, 2003
  54. Attentional set for external information activates the right intraparietal area
    Cognitive Brain Research, vol. 16, no. 2, pp. 199–209, 2003
  55. Discrete stochastic process underlying perceptual rivalry
    NeuroReport, vol. 14, no. 10, pp. 1347–1352, 2003
  56. Engagement of CD44 Promotes Rac Activation and CD44 Cleavage during Tumor Cell Migration
    Journal of Biological Chemistry, vol. 279, no. 6, pp. 4541–4550, 2003
  57. Model describing the biased Brownian movement of myosin.
    Proceedings of the Japan Academy, Series B, vol. 79B, no. 1, pp. 9–14, 2003
  58. Spectral Heterogeneity in Single Light-harvesting Chlorosomes from Green Sulfur Photosynthetic Bacterium Chlorobium tepidum¶
    Photochemistry and Photobiology, vol. 75, no. 4, p. 433, 2002
  59. Simultaneous Optical and Electrical Recording of a Single Ion-Channel.
    The Japanese Journal of Physiology, vol. 52, no. 5, pp. 429–434, 2002
  60. The motor domain determines the large step of myosin-V
    Nature, vol. 415, no. 6868, pp. 192–195, 2002
  61. Chemomechanical coupling of the forward and backward steps of single kinesin molecules
    Nature Cell Biology, vol. 4, no. 10, Article ID ncb857, 7 pages, 2002
  62. The Journal of Physical Chemistry B, vol. 106, no. 6, pp. 1430–1433, 2002
  63. Development of an Experimental Apparatus for Simultaneous Observation of Optical and Electrical Signals from Single Ion Channels
    Single Molecules, vol. 3, no. 1, pp. 33–42, 2002
  64. Detection of Thermal Processes of Protein Function
    Single Molecules, vol. 3, no. 2-3, pp. 149–153, 2002
  65. Class VI Myosin Moves Processively along Actin Filaments Backward with Large Steps
    Biochemical and Biophysical Research Communications, vol. 290, no. 1, pp. 311–317, 2002
  66. A new view concerning an actomyosin motor
    Cellular and Molecular Life Sciences, vol. 59, no. 11, pp. 1767–1770, 2002
  67. Cortical Mapping of Gait in Humans: A Near-Infrared Spectroscopic Topography Study
    NeuroImage, vol. 14, no. 5, pp. 1186–1192, 2001
  68. Non-Contact Electrostatic Surface Force Imaging of Single Protein Filaments using Intermolecular Force Microscopy
    Single Molecules, vol. 2, no. 3, pp. 183–190, 2001
  69. Substeps within the 8-nm step of the ATPase cycle of single kinesin molecules
    Nature Cell Biology, vol. 3, no. 4, Article ID 35070116, 3 pages, 2001
  70. Single molecule nanobioscience
    Trends in Biochemical Sciences, vol. 26, no. 7, pp. 438–444, 2001
  71. Single molecule nanomanipulation of biomolecules
    Trends in Biotechnology, vol. 19, no. 6, pp. 211–216, 2001
  72. Single-Molecule Analysis of Chemotactic Signaling in Dictyostelium Cells
    Science, vol. 294, no. 5543, pp. 864–867, 2001
  73. Single-Molecule Imaging of Interaction between Dextran and Glucosyltransferase from Streptococcus sobrinus
    Journal of Bacteriology, vol. 182, no. 4, pp. 1162–1166, 2000
  74. A large step for myosin
    Proceedings of the National Academy of Sciences, vol. 97, no. 17, pp. 9357–9359, 2000
  75. Electrophysiological Study of a Novel Large Pore Formed by Bax and the Voltage-dependent Anion Channel That Is Permeable to Cytochrome c
    Journal of Biological Chemistry, vol. 275, no. 16, pp. 12321–12325, 2000
  76. Single-motor mechanics and models of the myosin motor
    Philosophical Transactions of the Royal Society B: Biological Sciences, vol. 355, no. 1396, pp. 441–447, 2000
  77. Nature Cell Biology, vol. 2, no. 3, pp. 168–172, 2000
  78. Imaging And Nano-Manipulation Of Single Actomyosin Motors At Work
    Clinical and Experimental Pharmacology and Physiology, vol. 27, no. 3, pp. 229–237, 2000
  79. Single molecule analysis of the actomyosin motor
    Current Opinion in Cell Biology, vol. 12, no. 1, pp. 20–25, 2000
  80. Single-Molecule Imaging of Signaling Molecules in Living Cells
    Single Molecules, vol. 1, no. 2, pp. 159–163, 2000
  81. Single molecular observation of the interaction of GroEL with substrate proteins
    Journal of Molecular Biology, vol. 292, no. 5, pp. 965–972, 1999
  82. An Artificial Lipid Bilayer Formed on an Agarose-Coated Glass for Simultaneous Electrical and Optical Measurement of Single Ion Channels
    Biochemical and Biophysical Research Communications, vol. 265, no. 2, pp. 595–599, 1999
  83. Simultaneous Measurement of Individual ATPase and Mechanical Reactions by a Single Myosin Molecule at Work
    Optical Review, vol. 6, no. 1, pp. 16–23, 1999
  84. Biochemistry, vol. 38, no. 32, pp. 10318–10323, 1999
  85. Nature, vol. 397, no. 6715, pp. 129–134, 1999
  86. Fluorescence resonance energy transfer between single fluorophores attached to a coiled-coil protein in aqueous solution
    Chemical Physics, vol. 247, no. 1, pp. 163–173, 1999
  87. The gamma -subunit rotation and torque generation in F1-ATPase from wild-type or uncoupled mutant Escherichia coli
    Proceedings of the National Academy of Sciences, vol. 96, no. 14, pp. 7780–7784, 1999
  88. Single Molecule Imaging of Fluorescently Labeled Proteins on Metal by Surface Plasmons in Aqueous Solution
    Physical Review Letters, vol. 80, no. 20, pp. 4606–4609, 1998
  89. Filament structure as an essential factor for regulation of Dictyostelium myosin by regulatory light chain phosphorylation
    Proceedings of the National Academy of Sciences, vol. 95, no. 24, pp. 14124–14129, 1998
  90. Simultaneous Observation of Individual ATPase and Mechanical Events by a Single Myosin Molecule during Interaction with Actin
    Cell, vol. 92, no. 2, pp. 161–171, 1998
  91. Imaging and nano-manipulation of single biomolecules
    Biophysical Chemistry, vol. 68, no. 1-3, pp. 63–72, 1997
  92. Single molecular assay of individual ATP turnover by a myosin-GFP fusion protein expressed in vitro
    FEBS Letters, vol. 407, no. 2, pp. 235–238, 1997
  93. Non-contact scanning probe microscopy with sub-piconewton force sensitivity
    Ultramicroscopy, vol. 70, no. 1-2, pp. 45–55, 1997
  94. Myosin Subfragment-1 Is Fully Equipped with Factors Essential for Motor Function?
    Biochemical and Biophysical Research Communications, vol. 230, no. 1, pp. 76–80, 1997
  95. Subpiconewton Intermolecular Force Microscopy
    Biochemical and Biophysical Research Communications, vol. 231, no. 3, pp. 566–569, 1997
  96. Single Molecule Imaging of Fluorophores and Enzymatic Reactions Achieved by Objective-Type Total Internal Reflection Fluorescence Microscopy
    Biochemical and Biophysical Research Communications, vol. 235, no. 1, pp. 47–53, 1997
  97. Movements of truncated kinesin fragments with a short or an artificial flexible neck
    Proceedings of the National Academy of Sciences, vol. 94, no. 14, pp. 7275–7280, 1997
  98. Kinetics of force generation by single kinesin molecules activated by laser photolysis of caged ATP
    Proceedings of the National Academy of Sciences, vol. 94, no. 9, pp. 4395–4400, 1997
  99. Direct observation of single kinesin molecules moving along microtubules
    Nature, vol. 380, no. 6573, Article ID 380451a0, 2 pages, 1996
  100. Modification of the bi-directional sliding movement of actin filaments along native thick filaments isolated from a clam
    Journal of Muscle Research and Cell Motility, vol. 17, no. 6, pp. 637–646, 1996
  101. Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution
    Nature, vol. 374, no. 6522, Article ID 374555a0, 4 pages, 1995
  102. Single-Molecule Analysis of the Actomyosin Motor Using Nano-Manipulation
    Biochemical and Biophysical Research Communications, vol. 199, no. 2, pp. 1057–1063, 1994
  103. Force-Generating Domain of Myosin Motor
    Biochemical and Biophysical Research Communications, vol. 196, no. 3, pp. 1504–1510, 1993
  104. Nano-manipulation of actomyosin molecular motors in vitro: a new working principle
    Trends in Biochemical Sciences, vol. 18, no. 9, pp. 319–324, 1993
  105. Charge-Reversion Mutagenesis of Dictyostelium Actin to Map the Surface Recognized by Myosin During ATP-Driven Sliding Motion
    Proceedings of the National Academy of Sciences, vol. 90, no. 6, pp. 2127–2131, 1993
  106. Loose coupling between chemical and mechanical reactions in actomyosin energy transduction
    Advances in Biophysics, vol. 26, pp. 75–95, 1990
  107. Direct observation of molecular motility by light microscopy
    Cell Motility and the Cytoskeleton, vol. 10, no. 1-2, pp. 71–76, 1988
  108. Force measurements by micromanipulation of a single actin filament by glass needles
    Nature, vol. 334, no. 6177, Article ID 334074a0, 2 pages, 1988
  109. Sliding movement of single actin filaments on one-headed myosin filaments
    Nature, vol. 326, no. 6115, Article ID 326805a0, 3 pages, 1987
  110. Sliding distance of actin filament induced by a myosin crossbridge during one ATP hydrolysis cycle
    Nature, vol. 316, no. 6026, Article ID 316366a0, 3 pages, 1985
  111. Angle of active site of myosin heads in contracting muscle during sudden length changes
    Journal of Muscle Research and Cell Motility, vol. 6, no. 1, pp. 43–52, 1985
  112. Predominant attached state of myosin cross-bridges during contraction and relaxation at low ionic strength
    Journal of Molecular Biology, vol. 177, no. 4, pp. 769–785, 1984
  113. Direct observation of motion of single F-actin filaments in the presence of myosin
    Nature, vol. 307, no. 5946, Article ID 307058a0, 2 pages, 1984
  114. Studies on conformation of F-actin in muscle fibers in the relaxed state, rigor, and during contraction using fluorescent phalloidin
    The Journal of Cell Biology, vol. 97, no. 6, pp. 1663–1667, 1983
  115. Angles of nucleotides bound to cross-bridges in glycerinated muscle fiber at various concentrations of ?-ATP, ?-ADP and ?-AMPPNP detected by polarized fluorescence
    Journal of Molecular Biology, vol. 146, no. 4, pp. 539–560, 1981
  116. Conformational changes of F-actin-?-ADP in thin filaments in myosin-free muscle fibers induced by Ca2+
    Journal of Molecular Biology, vol. 140, no. 2, pp. 313–320, 1980
  117. Polarized fluorescence from ?-ADP incorporated into F-actin in a myosin-free single fiber: Conformation of F-actin and hanges induced in it by heavy meromyosin
    Journal of Molecular Biology, vol. 126, no. 3, pp. 507–524, 1978
  118. Effect of Myosin on Conformational Changes of F-Actin in Thin Filament in vivo Induced by Calcium Ions
    European Journal of Biochemistry, vol. 56, no. 2, pp. 547–556, 1975
  119. Conformational changes of F-actin in the thin filaments of muscle induced in Vivo and in Vitro by calcium ions
    Journal of Molecular Biology, vol. 90, no. 3, pp. 509–522, 1974