Peter Schuck

Peter Schuck received his Ph.D. degree in biophysics from the Johann-Wolfgang Goethe-Unversity Frankfurt, Germany, in 1994. For his dissertation, he was characterizing the oligomeric state and heterogeneous interactions of the detergent-solubilized band 3 protein from the erythrocyte membrane, and developing multi-wavelength analytical ultracentrifugation techniques. From 1994 to 1997, he was a Visiting Fellow in the laboratory of Dr. Allen Minton in the Laboratory of Biochemistry and Genetics, NIDDK, at the National Institutes of Health in Bethesda, where he worked on problems in optical biosensing and sedimentation velocity as tools for the study of protein interactions. From 1997 to 2003, he was a Visiting Associate and Staff Scientist in the Division of Bioengineering and Physical Science, OD, at the National Institutes of Health, developing and applying various biophysical methods for the study of macromolecular interactions. Since 2003, he is Chief of the Protein Biophysics Section, now located in LBPS at the National Institute of Biomedical Imaging and Bioengineering. Dr. Schuck is an Editorial Board member of The Protein Journal, Faculty Member in the Experimental Biophysical Methods section of the Structural Biology faculty of F1000 Biology, and editor of the book Protein Interactions - Biophysical Approaches for the Study of Complex Reversible Systems (Protein Reviews Vol. 5). He is organizer of the semiannual workshop on “Hydrodynamic and Thermodynamic Analysis of Macromolecules with sedfit and sedphat”, and author of a several widely used public domain computational biophysical analysis programs.

Biography Updated on 12 January 2008

Personal Home Page

http://www.nibib.nih.gov/Research/Intramural/LBPS/Staff/Schuck

Articles in Scholarly Journals [Incomplete List]

  1. A new adaptive grid-size algorithm for the simulation of sedimentation velocity profiles in analytical ultracentrifugation
    Computer Physics Communications, vol. 178, no. 2, pp. 105–120, 2008
  2. Single-walled tubulin ring polymers
    Biopolymers, vol. 86, no. 5-6, pp. 424–436, 2007
  3. Superantigen natural affinity maturation revealed by the crystal structure of staphylococcal enterotoxin G and its binding to T-cell receptor Vß8.2
    Proteins: Structure, Function, and Bioinformatics, vol. 68, no. 1, pp. 389–402, 2007
  4. Two Interferons Alpha Influence Each Other during Their Interaction with the Extracellular Domain of Human Type Interferon Receptor Subunit 2
    Biochemistry, vol. 46, no. 50, pp. 14638–14649, 2007
  5. Using Prior Knowledge in the Determination of Macromolecular Size-Distributions by Analytical Ultracentrifugation
    Biomacromolecules, vol. 8, no. 6, pp. 2011–2024, 2007
  6. Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1
    Nature Structural & Molecular Biology, vol. 13, no. 9, Article ID nsmb1133, 7 pages, 2006
  7. Chimpanzee/human mAbs to vaccinia virus B5 protein neutralize vaccinia and smallpox viruses and protect mice against vaccinia virus
    Proceedings of the National Academy of Sciences, vol. 103, no. 6, pp. 1882–1887, 2006
  8. Quaternary Structure and Cleavage Specificity of a Poxvirus Holliday Junction Resolvase
    Journal of Biological Chemistry, vol. 281, no. 17, pp. 11618–11626, 2006
  9. Efficient Neutralization of Anthrax Toxin by Chimpanzee Monoclonal Antibodies against Protective Antigen
    The Journal of Infectious Diseases, vol. 193, no. 5, pp. 625–633, 2006
  10. Studying multisite binary and ternary protein interactions by global analysis of isothermal titration calorimetry data in SEDPHAT: Application to adaptor protein complexes in cell signaling
    Protein Science, vol. 16, no. 1, pp. 30–42, 2006
  11. Protein S multimers and monomers each have direct anticoagulant activity
    Journal of Thrombosis and Haemostasis, vol. 4, no. 2, pp. 385–391, 2006
  12. Macromolecular Size-and-Shape Distributions by Sedimentation Velocity Analytical Ultracentrifugation
    Biophysical Journal, vol. 90, no. 12, pp. 4651–4661, 2006
  13. Probing the Functional Heterogeneity of Surface Binding Sites by Analysis of Experimental Binding Traces and the Effect of Mass Transport Limitation
    Biophysical Journal, vol. 92, no. 5, pp. 1742–1758, 2006
  14. Variable Dimerization of the Ly49A Natural Killer Cell Receptor Results in Differential Engagement of its MHC Class I Ligand
    Journal of Molecular Biology, vol. 362, no. 1, pp. 102–113, 2006
  15. Crystal Structure of Group A Streptococcus Mac-1: Insight into Dimer-Mediated Specificity for Recognition of Human IgG
    Structure, vol. 14, no. 2, pp. 225–235, 2006
  16. Targeted lysis of HIV-infected cells by natural killer cells armed and triggered by a recombinant immunoglobulin fusion protein: implications for immunotherapy
    Virology, vol. 332, no. 2, pp. 491–497, 2005
  17. Regulation of ASAP1 by phospholipids is dependent on the interface between the PH and Arf GAP domains
    Cellular Signalling, vol. 17, no. 10, pp. 1276–1288, 2005
  18. Biochemistry, vol. 44, no. 23, pp. 8470–8478, 2005
  19. Biochemistry, vol. 44, no. 39, pp. 13122–13131, 2005
  20. Sedimentation Velocity Analysis of Heterogeneous Protein-Protein Interactions: Lamm Equation Modeling and Sedimentation Coefficient Distributions c(s)
    Biophysical Journal, vol. 89, no. 1, pp. 619–634, 2005
  21. Sedimentation Velocity Analysis of Heterogeneous Protein-Protein Interactions: Sedimentation Coefficient Distributions c(s) and Asymptotic Boundary Profiles from Gilbert-Jenkins Theory
    Biophysical Journal, vol. 89, no. 1, pp. 651–666, 2005
  22. Characterization of DC-SIGN/R Interaction with Human Immunodeficiency Virus Type 1 gp120 and ICAM Molecules Favors the Receptor's Role as an Antigen-Capturing Rather than an Adhesion Receptor
    Journal of Virology, vol. 79, no. 8, pp. 4589–4598, 2005
  23. A Mechanism for Assembly of Complexes of Vitronectin and Plasminogen Activator Inhibitor-1 from Sedimentation Velocity Analysis
    Journal of Biological Chemistry, vol. 280, no. 31, pp. 28711–28720, 2005
  24. Dissection of Merozoite Surface Protein 3, a Representative of a Family of Plasmodium falciparum Surface Proteins, Reveals an Oligomeric and Highly Elongated Molecule
    Journal of Biological Chemistry, vol. 280, no. 44, pp. 37236–37245, 2005
  25. Studying multiprotein complexes by multisignal sedimentation velocity analytical ultracentrifugation
    Proceedings of the National Academy of Sciences, vol. 102, no. 1, pp. 81–86, 2005
  26. Exposure of acidic residues as a danger signal for recognition of fibrinogen and other macromolecules by integrin  X 2
    Proceedings of the National Academy of Sciences, vol. 102, no. 5, pp. 1614–1619, 2005
  27. Cooperative behavior of Escherichia coli cell-division protein FtsZ assembly involves the preferential cyclization of long single-stranded fibrils
    Proceedings of the National Academy of Sciences, vol. 102, no. 6, pp. 1895–1900, 2005
  28. Biochemistry, vol. 43, no. 14, pp. 4170–4178, 2004
  29. Hydrodynamics of Nanoscopic Tubulin Rings in Dilute Solutions
    Physical Review Letters, vol. 93, no. 9, 2004
  30. Crystal Structure of the C-terminal Peptidoglycan-binding Domain of Human Peptidoglycan Recognition Protein I 
    Journal of Biological Chemistry, vol. 279, no. 30, pp. 31873–31882, 2004
  31. SDR grafting of a murine antibody using multiple human germline templates to minimize its immunogenicity
    Molecular Immunology, vol. 41, no. 9, pp. 863–872, 2004
  32. Sedimentation equilibrium analysis of protein interactions with global implicit mass conservation constraints and systematic noise decomposition
    Analytical Biochemistry, vol. 326, no. 2, pp. 234–256, 2004
  33. A model for sedimentation in inhomogeneous media. I. Dynamic density gradients from sedimenting co-solutes
    Biophysical Chemistry, vol. 108, no. 1-3, pp. 187–200, 2004
  34. A model for sedimentation in inhomogeneous media. II. Compressibility of aqueous and organic solvents
    Biophysical Chemistry, vol. 108, no. 1-3, pp. 201–214, 2004
  35. Minimizing immunogenicity of the SDR-grafted humanized antibody CC49 by genetic manipulation of the framework residues
    Molecular Immunology, vol. 40, no. 6, pp. 337–349, 2003
  36. On the analysis of protein self-association by sedimentation velocity analytical ultracentrifugation
    Analytical Biochemistry, vol. 320, no. 1, pp. 104–124, 2003
  37. Inhibition of Hemostasis by a High Affinity Biogenic Amine-binding Protein from the Saliva of a Blood-feeding Insect
    Journal of Biological Chemistry, vol. 278, no. 7, pp. 4611–4617, 2003
  38. Alanine-scanning Mutations in Domain 4 of Anthrax Toxin Protective Antigen Reveal Residues Important for Binding to the Cellular Receptor and to a Neutralizing Monoclonal Antibody
    Journal of Biological Chemistry, vol. 278, no. 33, pp. 30936–30944, 2003
  39. Variable MHC class I engagement by Ly49 natural killer cell receptors demonstrated by the crystal structure of Ly49C bound to H-2Kb
    Nature Immunology, vol. 4, no. 12, Article ID ni1006, 9 pages, 2003
  40. Differences in the binding capacity of human apolipoprotein E3 and E4 to size-fractionated lipid emulsions
    European Journal of Biochemistry, vol. 269, no. 23, pp. 5939–5949, 2002
  41. Biochemical and Biological Characterization of a Dodecameric CD4-Ig Fusion Protein. IMPLICATIONS FOR THERAPEUTIC AND VACCINE STRATEGIES
    Journal of Biological Chemistry, vol. 277, no. 13, pp. 11456–11464, 2002
  42. Analysis of a Temperature-Sensitive Mutant Rotavirus Indicates that NSP2 Octamers Are the Functional Form of the Protein
    Journal of Virology, vol. 76, no. 14, pp. 7082–7093, 2002
  43. Modern analytical ultracentrifugation in protein science: A tutorial review
    Protein Science, vol. 11, no. 9, pp. 2067–2079, 2002
  44. Surface plasmon resonance-based competition assay to assess the sera reactivity of variants of humanized antibodies
    Journal of Immunological Methods, vol. 268, no. 2, pp. 197–210, 2002
  45. Biochemistry, vol. 41, no. 16, pp. 5177–5184, 2002
  46. Analytical Chemistry, vol. 74, no. 9, pp. 2041–2047, 2002
  47. Crystal Structure of a Superantigen Bound to the High-Affinity, Zinc-Dependent Site on MHC Class II
    Immunity, vol. 14, no. 1, pp. 93–104, 2001
  48. Analytical Chemistry, vol. 73, no. 13, pp. 2828–2835, 2001
  49. Rotavirus Nonstructural Protein NSP2 Self-assembles into Octamers That Undergo Ligand-induced Conformational Changes
    Journal of Biological Chemistry, vol. 276, no. 13, pp. 9679–9687, 2001
  50. Oligomeric structure of virion-associated and soluble forms of the simian immunodeficiency virus envelope protein in the prefusion activated conformation
    Proceedings of the National Academy of Sciences, vol. 98, no. 26, pp. 14877–14882, 2001
  51. Self-association of Human Apolipoprotein E3 and E4 in the Presence and Absence of Phospholipid
    Journal of Biological Chemistry, vol. 275, no. 47, pp. 36758–36765, 2000
  52. The Human Immunodeficiency Virus Type 1 gp120 V2 Domain Mediates gp41-Independent Intersubunit Contacts
    Journal of Virology, vol. 74, no. 10, pp. 4448–4455, 2000
  53. Biochemistry, vol. 39, no. 24, pp. 7291–7299, 2000
  54. Determination of the sedimentation coefficient distribution by least-squares boundary modeling
    Biopolymers, vol. 54, no. 5, pp. 328–341, 2000
  55. Analysis of Transport Experiments Using Pseudo-Absorbance Data
    Analytical Biochemistry, vol. 285, no. 1, pp. 135–142, 2000
  56. An antibody single-domain phage display library of a native heavy chain variable region: isolation of functional single-domain VH molecules with a unique interface
    Journal of Molecular Biology, vol. 290, no. 3, pp. 685–698, 1999
  57. Sedimentation Equilibrium Analysis of Interference Optical Data by Systematic Noise Decomposition
    Analytical Biochemistry, vol. 272, no. 2, pp. 199–208, 1999
  58. Characterizing the Solution Properties of Supramolecular Systems by Analytical Ultracentrifugation
    Chemistry - A European Journal, vol. 5, no. 5, pp. 1377–1383, 1999
  59. Sedimentation equilibrium analysis of recombinant mouse FcRn with murine IgG1
    Molecular Immunology, vol. 36, no. 15-16, pp. 1117–1125, 1999
  60. Salt Links Dominate Affinity of Antibody HyHEL-5 for Lysozyme through Enthalpic Contributions
    Journal of Biological Chemistry, vol. 274, no. 38, pp. 26838–26842, 1999
  61. Biochemistry, vol. 37, no. 46, pp. 16152–16164, 1998
  62. Determination of Binding Constants by Equilibrium Titration with Circulating Sample in a Surface Plasmon Resonance Biosensor
    Analytical Biochemistry, vol. 265, no. 1, pp. 79–91, 1998
  63. Reliable determination of binding affinity and kinetics using surface plasmon resonance biosensors
    Current Opinion in Biotechnology, vol. 8, no. 4, pp. 498–502, 1997
  64. USE OF SURFACE PLASMON RESONANCE TO PROBE THE EQUILIBRIUM AND DYNAMIC ASPECTS OF INTERACTIONS BETWEEN BIOLOGICAL MACROMOLECULES1
    Annual Review of Biophysics and Biomolecular Structure, vol. 26, no. 1, pp. 541–566, 1997
  65. Kinetic analysis of biosensor data: elementary tests for self-consistency
    Trends in Biochemical Sciences, vol. 21, no. 12, pp. 458–460, 1996
  66. Analysis of Mass Transport-Limited Binding Kinetics in Evanescent Wave Biosensors
    Analytical Biochemistry, vol. 240, no. 2, pp. 262–272, 1996