Francis J. Castellino
The overall interests of the laboratory are centered on the in vitro and in vivo relationships between hemostasis and inflammation. More specifically, the function and activation of proteins that participate in blood coagulation, anticoagulation, and blood clot dissolution are studied at the protein and gene levels using modern biochemical and biophysical techniques, e.g., cloning, mutagenesis and expression of variant recombinant proteins and individual protein domains, immunochemistry, and physical and chemical studies of their structures, employing X-ray crystallography, NMR, calorimetry. The properties of the proteins are then related to their functions. This work is then carried to the gene, cellular, and whole animal levels with the aim of understanding the cross-communications between the blood clotting system and the inflammatory response. Here, appropriate acute and chronic inflammatory disease models (e.g., sepsis/endotoxemia, atherosclerosis, asthma, cancer) are investigated in genetically-altered mice to attempt to mechanistically understand the pathophysiology of these diseases. Major tools employed in these studies include construction and utilization of in vivo gene-targeted and traditional transgenic mice, cell-specific gene microarray studies, organ- and cell-specific imaging, histology, and sophisticated mouse surgical approaches.
Biography Updated on 4 June 2009