H. Heng

Henry Heng is an Associate Professor for the Center for Molecular Medicine and Genetics of the Wayne State University Medical School. He is also an Associate Professor for the Karmanos Cancer Institute. His research has been focused on applying a genome-based system approach to evaluate the structure and function of genomes and their variations/abnormalities to determine the biological and clinical implications. By establishing the importance of nonclonal chromosomal aberrations (NCCAs), he has demonstrated that genomic instability mediated stochastic genome aberrations are the key driving force in cancer progression and genome variations play a dominant role in system instability and evolution. By applying this concept, his group has hypothesized that sexual reproduction promotes the continuation of a species by maintaining the chromosome defined framework of a species and that the main result of sexual reproduction is the preservation of the identity of a given genome. In addition to discovering a number of new forms of chromosome aberrations such as defective mitotic figures (DMFs) and chromosome fragmentation, his group has analyzed both meiotic and mitotic chromatin loops to illustrate novel features of the chromatin loop domain and genome architecture. His group is also well known for their seminal contributions of a number of visualization technologies including pioneering high resolution fiber FISH, multiple-color DNA-protein in situ codetection, and small-sized probe FISH detection on DAPI banded chromosomes. He has authored and coauthored over 150 publications and he currently serves on the editorial board of four international journals.

Biography Updated on 9 October 2007

Personal Home Page

http://cmmg.biosci.wayne.edu/faculty/heng/index.php

Articles in Scholarly Journals [Incomplete List]

  1. Cancer genome sequencing: The challenges ahead
    BioEssays, vol. 29, no. 8, pp. 783–794, 2007
  2. Dynamic stromal-epithelial interactions during progression of MCF10DCIS.com xenografts
    International Journal of Cancer, vol. 120, no. 10, pp. 2127–2134, 2007
  3. Elimination of altered karyotypes by sexual reproduction preserves species identity
    Genome, vol. 50, no. 5, pp. 517–524, 2007
  4. Mitotic Cell Death by Chromosome Fragmentation
    Cancer Research, vol. 67, no. 16, pp. 7686–7694, 2007
  5. Clonal and non-clonal chromosome aberrations and genome variation and aberration
    Genome, vol. 49, no. 3, pp. 195–204, 2006
  6. Combined multicolor-FISH and immunostaining
    Cytogenetic and Genome Research, vol. 114, no. 3-4, pp. 227–234, 2006
  7. Stochastic cancer progression driven by non-clonal chromosome aberrations
    Journal of Cellular Physiology, vol. 208, no. 2, pp. 461–472, 2006
  8. Heterogeneous Duplications in Patients with Pelizaeus-Merzbacher Disease Suggest a Mechanism of Coupled Homologous and Nonhomologous Recombination
    The American Journal of Human Genetics, vol. 77, no. 6, pp. 966–987, 2005
  9. ATM and p21 Cooperate to Suppress Aneuploidy and Subsequent Tumor Development
    Cancer Research, vol. 65, no. 19, pp. 8747–8753, 2005
  10. Overexpression of Cyclin D1 Promotes Tumor Cell Growth and Confers Resistance to Cisplatin-Mediated Apoptosis in an Elastase-myc Transgene-Expressing Pancreatic Tumor Cell Line
    Clinical Cancer Research, vol. 11, no. 16, pp. 6075–6086, 2005
  11. Sequential Molecular and Cellular Events during Neoplastic Progression: A Mouse Syngeneic Ovarian Cancer Model
    Neoplasia, vol. 7, no. 10, pp. 944–956, 2005
  12. Imaging genome abnormalities in cancer research
    Cell & Chromosome, vol. 3, no. 1, p. 1, 2004
  13. Chromatin loops are selectively anchored using scaffold/matrix-attachment regions
    Journal of Cell Science, vol. 117, no. 7, pp. 999–1008, 2004
  14. Complexity of CNC Transcription Factors As Revealed by Gene Targeting of the Nrf3 Locus
    Molecular and Cellular Biology, vol. 24, no. 8, pp. 3286–3294, 2004
  15. Male mouse meiotic chromosome cores deficient in structural proteins SYCP3 and SYCP2 align by homology but fail to synapse and have possible impaired specificity of chromatin loop attachment
    Cytogenetic and Genome Research, vol. 105, no. 2-4, pp. 182–188, 2004
  16. SV40 T antigen interacts with Nbs1 to disrupt DNA replication control
    Genes & Development, vol. 18, no. 11, pp. 1305–1316, 2004
  17. Corrigendum to “The hyaluronidase gene HYAL1 maps to chromosome 3p21.2–p21.3 in human and 9F1–F2 in mouse, a conserved candidate tumor suppressor locus” [Genomics 48 (1998) 63–70]
    Genomics, vol. 84, no. 1, p. 227, 2004
  18. From DNA structure to gene expression: mediators of nuclear compartmentalization and dynamics
    Chromosome Research, vol. 11, no. 5, pp. 435–445, 2003
  19. Analysis of marker or complex chromosomal rearrangements present in pre- and post-natal karyotypes utilizing a combination of G-banding, spectral karyotyping and fluorescence in situ hybridization
    Clinical Genetics, vol. 63, no. 5, pp. 358–367, 2003
  20. Human Chromosome 7: DNA Sequence and Biology
    Science, vol. 300, no. 5620, pp. 767–772, 2003
  21. Alternative promoters and polyadenylation regulate tissue-specific expression ofHemogen isoforms during hematopoiesis and spermatogenesis
    Developmental Dynamics, vol. 228, no. 4, pp. 606–616, 2003
  22. Circling, Deafness, and Yellow Coat Displayed by Yellow Submarine (Ysb) and Light Coat and Circling (Lcc) Mice with Mutations on Chromosome 3
    Genomics, vol. 79, no. 6, pp. 777–784, 2002
  23. Sperm nuclear matrix association of the PRM1->PRM2->TNP2 domain is independent of Alu methylation
    Molecular Human Reproduction, vol. 7, no. 10, pp. 903–911, 2001
  24. Association of deletions and translocation of the reduced folate carrier gene with profound loss of gene expression in methotrexate-resistant K562 human erythroleukemia cells
    Biochemical Pharmacology, vol. 61, no. 6, pp. 665–675, 2001
  25. Spectral karyotyping (SKY) of mouse meiotic chromosomes
    Genome, vol. 44, no. 2, pp. 293–298, 2001
  26. Re-defining the chromatin loop domain
    Cytogenetic and Genome Research, vol. 93, no. 3-4, pp. 155–161, 2001
  27. The combination of SKY and specific loci detection with FISH or immunostaining
    Cytogenetic and Genome Research, vol. 93, no. 3-4, pp. 195–202, 2001
  28. Spectral karyotyping of mouse cell line WMP2
    Cytogenetic and Genome Research, vol. 90, no. 3-4, pp. 271–274, 2000
  29. Cloning and characterization of additional members of the G protein-coupled receptor family
    Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, vol. 1490, no. 3, pp. 311–323, 2000
  30. Identification of the mouse β`-COP golgi component as a spermatocyte autoantigen in scleroderma and mapping of its gene Copb2 to mouse Chromosome 9
    Cytogenetic and Genome Research, vol. 87, no. 3-4, pp. 201–204, 1999
  31. Identification of a Human Histone Acetyltransferase Related to Monocytic Leukemia Zinc Finger Protein
    Journal of Biological Chemistry, vol. 274, no. 40, pp. 28528–28536, 1999
  32. Identification and cloning of three novel human G protein-coupled receptor genes GPR52, ?GPR53 and GPR55: GPR55 is extensively expressed in human brain
    Molecular Brain Research, vol. 64, no. 2, pp. 193–198, 1999
  33. Nontargeted Stable Integration of Recombinant Adeno-Associated Virus into Human Leukemia and Lymphoma Cell Lines as Evaluated by Fluorescence in Situ Hybridization
    Human Gene Therapy, vol. 10, no. 4, pp. 537–543, 1999
  34. Discovery of Three Novel Orphan G-Protein-Coupled Receptors
    Genomics, vol. 56, no. 1, pp. 12–21, 1999
  35. Cloning and Expression Analysis of a Novel WD Repeat Gene, WDR3, Mapping to 1p12–p13
    Genomics, vol. 59, no. 1, pp. 85–89, 1999
  36. The Hyaluronidase Gene HYAL1 Maps to Chromosome 3p21.2–p21.3 in Human and 9F1–F2 in Mouse, a Conserved Candidate Tumor Suppressor Locus,
    Genomics, vol. 48, no. 1, pp. 63–70, 1998
  37. Discovery of Three Novel G-Protein-Coupled Receptor Genes
    Genomics, vol. 47, no. 2, pp. 310–313, 1998
  38. Mapping of the HumanHPRP3andHPRP4Genes Encoding U4/U6-Associated Splicing Factors to Chromosomes 1q21.1 and 9q31–q33,
    Genomics, vol. 48, no. 2, pp. 273–275, 1998
  39. Cloning Genes Encoding Receptors Related to Chemoattractant Receptors
    Genomics, vol. 50, no. 2, pp. 281–286, 1998
  40. Aquarius, a novel gene isolated by gene trapping with an RNA-dependent RNA polymerase motif
    Developmental Dynamics, vol. 212, no. 2, pp. 304–317, 1998
  41. Chromosomal mapping and expression of the human B120 gene
    Gene, vol. 213, no. 1-2, pp. 189–193, 1998
  42. Assignment of the Gene Encoding the Limbic System-Associated Membrane Protein (LAMP) to Mouse Chromosome 16B5 and Human Chromosome 3q13.2–q21?
    Genomics, vol. 49, no. 3, pp. 472–474, 1998
  43. Structure and mapping of the human ß-defensin HBD-2 gene and its expression at sites of inflammation
    Gene, vol. 222, no. 2, pp. 237–244, 1998
  44. Assignment of Mouse Fibroblast Growth Factor 10 (Fgf10) Gene to the Telomeric Region of Chromosome 13
    Genomics, vol. 53, no. 2, pp. 247–248, 1998
  45. Cloning and mapping of a cDNA for methionine synthase reductase, a flavoprotein defective in patients with homocystinuria
    Proceedings of the National Academy of Sciences, vol. 95, no. 6, pp. 3059–3064, 1998
  46. High resolution free chromatin/DNA fiber fluorescent in situ hybridization
    Journal of Chromatography A, vol. 806, no. 1, pp. 219–229, 1998
  47. Overlapping and non-overlapping Ptch2 expression with Shh during mouse embryogenesis
    Mechanisms of Development, vol. 78, no. 1-2, pp. 81–84, 1998
  48. Rad51 immunocytology in rat and mouse spermatocytes and oocytes
    Chromosoma, vol. 106, no. 4, pp. 207–215, 1997
  49. Cloning of a DSS1 pseudogene (DSS1P1) and mapping to human Chromosome band 5q14
    Mammalian Genome, vol. 8, no. 2, pp. 159–160, 1997
  50. Fish technology in chromosome and genome research
    BioEssays, vol. 19, no. 1, pp. 75–84, 1997
  51. The Human ß-Defensin-1 and a-Defensins Are Encoded by Adjacent Genes: Two Peptide Families with Differing Disulfide Topology Share a Common Ancestry,
    Genomics, vol. 43, no. 3, pp. 316–320, 1997
  52. Assignment of the Human dUTPase Gene (DUT) to Chromosome 15q15–q21.1 by Fluorescencein SituHybridization
    Genomics, vol. 40, no. 1, pp. 213–215, 1997
  53. Expression Analysis and Chromosomal Assignment of the HumanSFRS5/SRp40Gene
    Genomics, vol. 43, no. 2, pp. 165–170, 1997
  54. Cloning and chromosomal mapping of four putative novel human G-protein-coupled receptor genes
    Gene, vol. 187, no. 1, pp. 75–81, 1997
  55. Genomic Organization and Functional Analysis of the Murine Protein Phosphatase 1c ? (Ppp1cc) Gene
    Genomics, vol. 45, no. 1, pp. 211–215, 1997
  56. Discovery of a Novel Human G Protein-Coupled Receptor Gene (GPR25) Located on Chromosome 1
    Biochemical and Biophysical Research Communications, vol. 230, no. 1, pp. 69–72, 1997
  57. PMS2-Related Genes Flank the Rearrangement Breakpoints Associated with Williams Syndrome and Other Diseases on Human Chromosome 7
    Genomics, vol. 45, no. 2, pp. 402–406, 1997
  58. Amplification of CFTR Exon 9 Sequences to Multiple Locations in the Human Genome
    Genomics, vol. 45, no. 3, pp. 554–561, 1997
  59. Identification and characterization of human genes encoding Hprp3p and Hprp4p, interacting components of the spliceosome
    Human Molecular Genetics, vol. 6, no. 12, pp. 2117–2126, 1997
  60. Regulation of meiotic chromatin loop size by chromosomal position
    Proceedings of the National Academy of Sciences, vol. 93, no. 7, pp. 2795–2800, 1996
  61. Localization of a novel zinc finger gene to the human chromosome 7p11.2-p12 by fluorescencein situ hybridization
    Somatic Cell and Molecular Genetics, vol. 22, no. 3, pp. 237–239, 1996
  62. Localization of a novel zinc finger gene to human chromosome 7q22 region by fluorescencein situ hybridization
    Somatic Cell and Molecular Genetics, vol. 22, no. 3, pp. 233–235, 1996
  63. Direct detection of expanded trinucleotide repeats using PCR and DNA hybridization techniques
    American Journal of Medical Genetics, vol. 67, no. 1, pp. 85–91, 1996
  64. The Genes for the a and ß Subunits of the Mitochondrial Trifunctional Protein Are Both Located in the Same Region of Human Chromosome 2p23
    Genomics, vol. 37, no. 1, pp. 141–143, 1996
  65. Localization of three novel zinc finger genes to the centromeric region of human chromosome 10 by fluorescencein situ hybridization
    Somatic Cell and Molecular Genetics, vol. 22, no. 3, pp. 241–244, 1996
  66. Localization of Two Metabotropic Glutamate Receptor Genes,GRM3andGRM8, to Human Chromosome 7q
    Genomics, vol. 31, no. 2, pp. 230–233, 1996
  67. Tissue Distribution, Genomic Structure, and Chromosome Mapping of Mouse and Human Eukaryotic Initiation Factor 4E-Binding Proteins 1 and 2
    Genomics, vol. 38, no. 3, pp. 353–363, 1996
  68. Physical Mapping of Receptor Type Protein Tyrosine Phosphatase Sigma (PTPRS) to Human Chromosome 19p13.3
    Genomics, vol. 38, no. 1, pp. 76–78, 1996
  69. Identification of Genes from a 500-kb Region at 7q11.23 That Is Commonly Deleted in Williams Syndrome Patients
    Genomics, vol. 36, no. 2, pp. 328–336, 1996
  70. A Novel Human Gene Encoding a G-Protein-Coupled Receptor (GPR15) Is Located on Chromosome 3
    Genomics, vol. 32, no. 3, pp. 462–465, 1996
  71. Identification of Sonic hedgehog as a candidate gene responsible for holoprosencephaly
    Nature Genetics, vol. 14, no. 3, Article ID ng1196-353, 3 pages, 1996
  72. Identification of a locus of zinc finger genes in human chromosome 19q13.1-q13.3 region by fluorescencein situ hybridization
    Somatic Cell and Molecular Genetics, vol. 22, no. 3, pp. 245–248, 1996
  73. A novel family of repeat sequences in the mouse genome responsive to retinoic acid
    Mammalian Genome, vol. 7, no. 10, pp. 741–748, 1996
  74. Chromosomal mapping of the second human CD8B gene locus
    Immunogenetics, vol. 43, no. 4, pp. 220–226, 1996
  75. A novel gene codes for a putative G protein-coupled receptor with an abundant expression in brain
    FEBS Letters, vol. 394, no. 3, pp. 325–329, 1996
  76. Characterization of a human gene related to genes encoding somatostatin receptors
    FEBS Letters, vol. 398, no. 2-3, pp. 253–258, 1996
  77. Genomic Organization and FISH Mapping of Human Pmel 17, the Putative Silver Locus
    Pigment Cell Research, vol. 9, no. 1, pp. 42–48, 1996
  78. Sequence Analysis and Chromosomal Localization of Human Cap Z
    Journal of Biological Chemistry, vol. 270, no. 37, pp. 21472–21479, 1995
  79. Cloning, Expression, and Chromosomal Localization of the Human Uridine Nucleotide Receptor Gene
    Journal of Biological Chemistry, vol. 270, no. 52, pp. 30845–30848, 1995
  80. Assignment of the Human GABA Transporter Gene (GABATHG) Locus to Chromosome 3p24-p25
    Genomics, vol. 29, no. 1, pp. 302–304, 1995
  81. Cloning and Chromosomal Mapping of Three Novel Genes, GPR9, GPR10, and GPR14, Encoding Receptors Related to Interleukin 8, Neuropeptide Y, and Somatostatin Receptors
    Genomics, vol. 29, no. 2, pp. 335–344, 1995
  82. Mapping of a Human LIM Protein (CLP) to Human Chromosome 11p15.1 by Fluorescence in Situ Hybridization
    Genomics, vol. 28, no. 3, pp. 602–603, 1995
  83. Structure of the Murine MPTP-PEST Gene: Genomic Organization and Chromosomal Mapping
    Genomics, vol. 28, no. 3, pp. 501–507, 1995
  84. The Cloning and Chromosomal Mapping of Two Novel Human Opioid-Somatostatin-like Receptor Genes, GPR7 and GPR8, Expressed in Discrete Areas of the Brain
    Genomics, vol. 28, no. 1, pp. 84–91, 1995
  85. Assignment of the human homolog of mouse Dlx3 to Chromosome 17q21.3-q22 by analysis of somatic cell hybrids and fluorescence in situ hybridization
    Mammalian Genome, vol. 6, no. 4, pp. 310–311, 1995
  86. Regional Localization of 725 Human Chromosome 7-Specific Yeast Artificial Chromosome Clones
    Genomics, vol. 22, no. 2, pp. 439–448, 1994
  87. Cloning of Human Genes Encoding Novel G Protein-Coupled Receptors
    Genomics, vol. 23, no. 3, pp. 609–618, 1994
  88. Localization of the Human Gene for Inducible Nitric Oxide Synthase (NOS2) to Chromosome 17q11.2-q12
    Genomics, vol. 19, no. 1, pp. 183–185, 1994
  89. Organization of heterologous DNA inserts on the mouse meiotic chromosome core
    Chromosoma, vol. 103, no. 6, pp. 401–407, 1994
  90. The murine Xe169 gene escapes X–inactivation like its human homologue
    Nature Genetics, vol. 7, no. 4, Article ID ng0894-491, 5 pages, 1994
  91. Isolation of P1 bacteriophage clones containing large contiguous segments of the human and mouse loci for the T-cell coreceptor molecule CD8
    Genetic Analysis: Biomolecular Engineering, vol. 11, no. 5-6, pp. 129–139, 1994
  92. A human gene that shows identity with the gene encoding the angiotensin receptor is located on chromosome 11
    Gene, vol. 136, no. 1-2, pp. 355–360, 1993
  93. Modes of DAPI banding and simultaneous in situ hybridization
    Chromosoma, vol. 102, no. 5, pp. 325–332, 1993
  94. Chromosomal Localization of the Human Gene Encoding the 51-kDa Subunit of Mitochondrial Complex I (NDUFV1) to 11q13
    Genomics, vol. 18, no. 2, pp. 435–439, 1993
  95. Refined Mapping of the GM2 Activator Protein (GM2A) Locus to 5q31.3-q33.1, Distal to the Spinal Muscular Atrophy Locus
    Genomics, vol. 18, no. 2, pp. 429–431, 1993
  96. High-Resolution Mapping of Mammalian Genes by In situ Hybridization to Free Chromatin
    Proceedings of the National Academy of Sciences, vol. 89, no. 20, pp. 9509–9513, 1992