Effect of neurotoxin and H. partita venom on frog’s sciatic nerve gastrocnemius muscle preparation. Sciatic nerve gastrocnemius muscle was indirectly stimulated at 5 min intervals by single impulse (1 ms duration). Different concentrations of neurotoxin (0 [-■-], 10 [-▲-], 20 [-◆-], 30 [-○-], and 40 [-x-] μg/mL) (solid lines) and H. partita whole venom 40 [--] μg/mL (broken line) added to Ringer’s solution in organ bath, the twitching was followed independently, and the twitch height was recorded. Each point represents mean ± SD of three independent experiments. *indicates significant differences compared to control group as determined by one-way ANOVA, DMRT test at .