- About this Journal
- Abstracting and Indexing
- Aims and Scope
- Article Processing Charges
- Articles in Press
- Author Guidelines
- Bibliographic Information
- Citations to this Journal
- Contact Information
- Editorial Board
- Editorial Workflow
- Free eTOC Alerts
- Publication Ethics
- Reviewers Acknowledgment
- Submit a Manuscript
- Table of Contents
Case Reports in Medicine
Volume 2013 (2013), Article ID 740837, 12 pages
Behçet’s Disease, Associated Large Vessel Thrombosis, and Coexistent Thrombophilia: A Distinct Nosological Entity?
1st Department of Internal Medicine, “Georgios Papanikolaou” General Hospital, Exochi, 570 10 Thessaloniki, Greece
Received 15 February 2013; Revised 22 May 2013; Accepted 10 June 2013
Academic Editor: Christos D. Lionis
Copyright © 2013 Dimitrios Stoimenis et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Behçet’s disease (BD) represents a multisystemic disorder that combines features of immune-mediated diseases and autoinflammatory disorders. Even though it is recognized that every type or size of vessel can be affected in this disease, there is an inability to describe a coherent model that sufficiently explains the predilection of certain patients with BD for manifesting severe large vessel thrombosis. The inconsistent epidemiologic data and the complex genetic background of BD, along with the controversy of multiple international studies regarding the coexistence of thrombophilia in patients with BD and large vessel thrombosis, make us think that a percentage of these patients may actually suffer from a distinct clinical entity. The stimulus for this concept arose from the clinical observation of three male patients who were admitted to our clinic due to extended vena cava thrombosis. On the occasion of those clinically and laboratory resembling cases, we performed a literature review concerning the epidemiology of BD, associated thrombosis, and coexistent thrombophilic factors, in order to present some evidence, which sustains our hypothesis that certain patients with large vessel thrombosis, who share features of BD and coexistent thrombophilia, should actually be further investigated for the possibility of suffering from a distinct nosological entity.
Behçet’s disease (BD), which is also acknowledged as Adamantiades-Behçet’s disease, is classically defined as a chronic multisystemic vasculitis affecting vessels of any type or size and it is mostly characterized by recurrent mucocutaneous and ocular lesions. Over the last few years, the studies conducted on the pathophysiology of BD demonstrated its common characteristics with the group of autoinflammatory diseases. As a result, nowadays, many authors prefer to use an alternative term that defines BD as a chronic, relapsing, and multisystem autoinflammatory disorder of unknown etiology . Despite the international concern and the copious researches on BD, no pathognomonic laboratory finding or diagnostic test has yet been validated and the diagnosis of the disease remains a clinical decision. Over 15 different sets of criteria for the diagnosis of BD have been proposed but the most commonly used are the criteria of the International Study Group (ISG) which were created in 1990  and the Japanese criteria that were revised by the Behçet’s Disease Research Committee of Japan in 2003 [3, 4].
According to the ISG criteria, the diagnosis of BD requires the presence of recurrent oral ulceration plus any two of genital ulceration, typical defined eye lesions, typical defined skin lesions, or a positive pathergy test (Table 1). In one study of pediatric BD, the average period length between the initial oral ulceration and the second major manifestation was 8.8 years . The Japanese criteria are divided into major and minor manifestations and were created in order to avoid overdiagnosis of BD. The disease is classified as complete, incomplete, possible, or specific type, based on the combination of those manifestations (Table 2). The ISG criteria emphasize the oral aphthosis and a positive pathergy test, but they do not consider other important site-specific manifestations such as the vascular lesions, which in certain cases can be the initial presentation of BD . On the other hand, the Japanese criteria embody most of the site-specific manifestations, denominating three particular types of BD (neuro-, intestinal-, and vasculo-BD), but the diagnosis of the complete type of BD requires the presence of all the 4 predominant manifestations of BD. Therefore, although both sets of diagnostic criteria provide a useful diagnostic tool for the clinicians, they cannot ensure case ascertainment.
BD presents a significant inhomogeneity in clinical features, in the reported prevalences of the specific types, in gender predominance, and in genetic and immunological findings, vastly depending on which geographic region or ethnicity is studied. Thereafter, the inconsistent presentation of BD raises the question whether this is the result of a multifactorial interaction or if there is a possibility that what we call Behçet’s disease is to be constituted by a group of overlapping separate diseases. In this paper, we initially describe three cases with major vein thrombosis from which the first was diagnosed as complete vasculo-BD, the second was diagnosed as possible vasculo-BD, and the third was diagnosed as deep vein thrombosis (DVT) due to multifactorial thrombophilia. On the occasion of those three clinically and laboratory resembling cases, we performed a literature review concerning the epidemiology of BD, associated thrombosis, and coexistent thrombophilic factors, in order to support our hypothesis that BD might not represent a unique clinical entity and that in certain patients with large vessel thrombosis, who present some features of BD and coexistent thrombophilic factors, the possibility of suffering from a distinct nosological entity should be further investigated.
2. Subjects and Methods
Three cases with vena cava thrombosis were studied. Demographic characteristics, clinical signs and symptoms, radiologic imaging, laboratory and thrombophilic testing, treatment, and outcome were recorded.
Three adult Caucasian males of Greek origin aged 32, 54, and 20 (patients A, B, and C, resp.) were admitted to our Department of Internal Medicine due to large vessel thrombosis. All of the three patients had free medical history.
3.1. Clinical, Laboratory, and Imaging Assessments
Patient A. A 32-year-old male was admitted to our department with complaints of dizziness, headache, shortness of breath, and persisting low-grade fever starting a month ago. On initial evaluation, the patient had facial edema and distended jugular and upper chest veins. Additionally, tongue, gingival, and scrotal aphthous ulcers were observed. The patient confirmed the presence of recurrent oral aphthosis the last semester, which was painful and self-limited. A nodular episcleritis of the left eye was observed during ocular examination and pathergy test was positive, while no other cutaneous manifestations were observed. Digital subtraction angiography revealed an extended thrombosis of the superior vena cava (SVC) and the right subclavian vein and the creation of a collateral pathway through the azygos vein (Figure 1). Further evaluation with a transesophageal echocardiogram (triplex) showed a sizeable (3 cm) pedunculated thrombus, which was oscillating in the right atrium (Figure 2). Laboratory findings were characterized by moderately elevated inflammatory markers and mild normochromic anemia, while the immunological tests identified the HLA-B*51 haplotype and enhanced C4 complement titer, with negative results for the autoantibodies testing (Table 3). The patient was assessed for thrombophilic factors and was found to be homozygous carrier for the C677T polymorphism of the 5,10-methylene-tetrahydro-folate reductase (MTHFR) mutation, but serum homocysteine was within the normal range (Table 4).
Patient B. A 54-year-old male was referred to our department from a vascular surgery clinic due to a recently diagnosed inferior vena cava thrombosis. Patient’s history was remarkable for a 7-month bilateral and gradually ascending deep vein thrombosis of the lower limps; despite the fact that ever since he was diagnosed with the initiatory left popliteal vein thrombosis, he was treated with low molecular weight heparin (LMWH), used at therapeutic dose. Clinically, the patient had painful aphthous ulcers on the lips, gingiva, and oral cavity, characterized by frequent recurrence over the past 2 years. An ocular test was unremarkable; however, the pathergy test was positive although no other cutaneous lesions were observed. The patient had had triplex ultrasound repeated over the last months, which showed the bilateral and scaling course of the thrombosis of the large vessels of the lower limps, the iliac and the femoral veins (Figure 3), and the inferior vena cava. A computed tomography scan with IV contrast confirmed the sonographic findings. Laboratory assessment revealed elevated inflammatory markers and C3 complement titer, mild normochromic anemia, and positive HLA-B*51 type, while the results of the autoantibodies were all negative (Table 3). Moreover, the patient was found to be positive in several thrombophilic factors as he was heterozygous carrier of MTHFR C677T and FV-Leiden R506Q mutations with associated severe hyperhomocysteinemia (Table 4).
Patient C. A 20-year-old male was admitted to our department for a three-week continuous low back and thigh pain, accompanied by local tenderness, warmth and erythema, and a two-week fever. One month before his admission, the patient had been hospitalized in a vascular surgery clinic, where the diagnosis of inferior vena cava thrombosis was made, with an expansion of the thrombus to the ileac and the femoral veins bilaterally. He had been discharged with LMWH, prescribed at therapeutic dose. On examination, he was febrile up to 38.3°C and tenderness was present in iliac fossa and thigh bilaterally and especially left, with no other notable findings. The oral cavity and scrotum were free of any aphthous or herpetiform ulcers and cutaneous manifestations were absent; however, the patient mentioned the occurrence of two episodes of painless, self-limited aphthous ulcers in the last 9 months. An ocular test was unremarkable and the pathergy test was negative. A CT angiography was performed showing unchanged thrombosis of the inferior vena cava, ileac, spermatic, and femoral vein and the creation of a collateral pathway, along with a significant degree of perivascular inflammation (Figure 4). Laboratory exams revealed highly elevated inflammatory markers, slightly enhanced IgA and C4 complement titer, mild normochromic anemia, and prolonged partial thromboplastin time as well as the identification of HLA-B*51 and B*27 haplotypes (Table 3). Screening for autoantibodies was negative. Thrombophilia testing showed a positive low titer lupus anticoagulant (LAC) and a borderline hyperhomocysteinemia, while the patient was also found to be heterozygous carrier of MTHFR C677T and FV-Leiden R506Q mutations (Table 4).
3.2. Differential Diagnosis
In all the three cases, no evidence of infection, an underlying malignancy, or any other systemic vasculitis was documented by the clinical, imaging, and laboratory assessments. The main diagnostic findings of the three patients are summarized in Table 5.
Patient A. The occurrence of recurrent oral aphthosis, genital ulceration, and a positive pathergy test established the diagnosis of BD according to the ISG criteria. It is worth noting that his ocular finding (unilateral nodular episcleritis) has been reported as a rare BD finding but it is not included in the eye lesions in the diagnostic sets of criteria for BD.
Patient B. Hisclinical assessment included recurrent oral aphthosis and a positive pathergy test, which could not establish Behçet’s diagnosis according to the ISG criteria, but the implementation of the Japanese criteria of 2003 could not exclude a Behçet’s diagnosis in this case. HLA-B*51 positivity, acute phase response, and predominantly the deteriorating vascular lesions, even though he was receiving antithrombotic therapy with LMWH at therapeutic dose, supported the diagnosis of a possible type of BD. The main differential diagnosis in this patient was paraneoplastic thrombosis but the failure of the 7-month LMWH therapy in combination with the extended CT scanning and the gastrointestinal endoscopy did not provide evidence of any underlying malignancy. Likewise, it is doubtful whether the coexistence of three thrombophilic factors can itself explain his deteriorating clinical course, considering he was under sufficient antithrombotic therapy.
Patient C. His diagnostic assessment was remarkable for the coexistence of several different thrombophilic factors. A possible diagnosis of antiphospholipid syndrome was assumed but the reexamination in 6 and 12 weeks later did not detect the lupus anticoagulant. From the clinical assessment, even though he mentioned recurrent oral aphthosis, this manifestation was not observed by any physician and could not be evaluated. His laboratory findings were noticeable for the acute phase response and positivity in HLA-B*51&B*27, which, combined with the intense perivascular inflammation in CT angiography, posed the question whether all these findings were the result of the multifactorial thrombophilia and the extended thrombosis alone or if another inflammatory disorder was present. A biopsy of the small saphenous vein was performed and the pathology report described normal findings with no presence of inflammation or clot formation. The patient was also tested for JAK mutations with negative results.
3.3. Treatment and Outcome
Patient A. He was treated with oral immunosuppressants (methylprednisolone plus azathioprine) and anticoagulant agents (LMWH was administered at therapeutic dose for 3 days, followed by acenocoumarol with an INR target of 3–3.5). A successful surgical removal of the right atrium thrombus was performed, along with an SVC and brachiocephalic vein biopsy. The pathology report of those specimens described clot in incipient organization, inflammatory, and reactive lesions, findings which were compatible with vasculitis. He was discharged with a prescription of oral azathioprine, methylprednisolone with tapering dosage, aspirin, acenocoumarol, folic acid, and colchicine only during the occurrence of oral ulcerations. Clinical improvement was obtained in 1 month with a decrease of the inflammatory markers within the normal range. In a period of 6 months, the thromboses partially subsided, with complete resolution of the thrombi and vessel recanalization in 24 months. No signs of a relapse were observed during the 11-year follow-up, apart from the sporadic oral aphthosis.
Patient B. On admission, LMWH was discontinued and he was given acenocoumarol therapy with an INR target of 3–3.5. Soon after the completion of the initial diagnostic procedures, oral immunosuppressants (methylprednisolone plus azathioprine), colchicine, and folic acid per os were added in his medication. One month later, significant clinical improvement was observed with a decrease in the inflammatory markers within normal range. He was discharged with a prescription of oral azathioprine, methylprednisolone with tapering dosage, acenocoumarol, folic acid, and colchicine only during the occurrence of oral ulcerations. CT angiography of the IVC was performed in the 2-month follow-up revealing considerable remission of the thromboses with sufficient blood flow and partial recanalization of the IVC and the iliac veins. Complete clinical remission was observed in the total 6-month follow-up.
Patient C. He was treated with anticoagulant agents, which included LMWH at therapeutic dose followed by oral acenocoumarol with an INR target of 3–3.5. Folic acid was also added to his medication. Patient’s inflammatory markers gradually decreased within normal range, and the fever subsided during his hospitalization without any anti-inflammatory medication. A CT angiography prior to his discharge showed the subsidence of the perivascular inflammation but no improvement of the thrombosis. Patient’s assessment did not confirm any of the hallmark manifestations of BD, and he was discharged with a prescription of oral acenocoumarol. In the total 7-month follow-up, the patient presented distention of the abdominal veins due to the IVC obstruction, while his repeated imaging assessment, which included ultrasonography, computed tomography angiography, and magnetic resonance venography, has not shown sufficient subsidence of the DVT and IVC thrombosis. During the follow-up, examination for lupus anticoagulant was negative and no sign of any underlying malignancy or systemic vasculitis was evidenced from the elaborate clinical, laboratory, and imaging assessment.
4.1. Epidemiology of Behçet’s Disease
Although there is evidence for a global incidence, BD is more prevalent in the Far East, Middle East, and Eastern Mediterranean basin, following the pattern of the old silk trading routes, whereas it is rarely encountered in Northern Europe, Northern Asia, Sub-Saharan Africa, and America . The highest prevalence of BD is observed in Turkey with 420 cases per 100,000 population, while reported estimates vary from 13.5–22 cases per 100,000 population in Japan, Korea, China, Iran, and Saudi Arabia , and between 0.12 and 7.5 per 100,000 population in Europe and the US . However, the epidemiology cannot be scaled with accuracy, since the worldwide reported prevalences of BD are strongly influenced by the miscellaneous diagnostic sets of criteria that are applied in every country. There is a general estimation that BD occurs roughly equally in both sexes, with a worse prognosis and a more severe course of the disease in men . Nevertheless, there are several reports showing a divergent gender predominance of BD [20, 21]. In the Middle East, there are reports indicating a male predominance of BD, with male-to-female ratios of 3.8 : 1 in Israel and 5.3 : 1 in Egypt . Quite the opposite, in the United States there is a predominance of the females, with a female-to-male ratio of 5 : 1, whilst in Germany, Japan, and Brazil, the disease is slightly more common in females . As far as the age of BD onset is concerned, the symptoms typically occur in the late third and early fourth decades of life . Juvenile BD is rather unusual, representing 3% to 7% of all cases .
The pathogenesis of the disease remains uncertain. It is believed that genetic, autoimmune end environmental factors interact with each other. Historically, the most consistent association is with HLA-B*51 gene (chromosome 6p21) although the third of BD patients do not possess this gene . The geographic distribution of this gene is concomitant to the prevalence of BD in specific latitudes, namely, between 30° and 45° North . In particular, in countries in the southern hemisphere and in Europe above 45° North the prevalence of HLA-B*51 in healthy individuals is nil or low (1–10%), in countries in Central Europe its prevalence is intermediate (11–15%), and in countries in the eastern Mediterranean basin and in Middle East its prevalence is high (>15%) . Other MHC associations with BD include tumor necrosis factor (TNF), the heat shock protein (HSP) family, and the MHC class I chain gene A (MICA) . Moreover, 16 different non-HLA susceptibility loci were identified in whole-genome screening as being associated with the disease . The environmental factors that have been most implicated in triggering the inception of BD are infectious agents such as Herpes simplex virus type 1 (HSV-1), Streptococcus sanguinis, S. oralis, S. mitis, and Saccharomyces cerevisiae [19, 23, 25].
The most intriguing feature in the epidemiologic study of BD concerns the regional variation of its clinical expression, especially regarding the neurological, vascular, and intestinal manifestations of BD. In the case of neuro-BD, the reported percentages range from 1.3% to 59% in hospital-based series and 5.3% to 14.3% in three prospective studies from Turkey, Iran, and Iraq . Similarly, gastrointestinal involvement in BD has been frequently reported in Japan (50–60%), United Kingdom (38–53%), and Taiwan (32%), whereas it is rare in Turkey (3%), Saudi Arabia (4%), and Jordan (5%) . Lastly, the same disparity is presented in the reported prevalences of vascular BD, which is frequent in Saudi Arabia (34%)  and Turkey (14.3–26%) [29, 30] and lower in Iran (8.3%)  and Japan (6%) . However, we must notice that the lack of case definition can partially explain the deviant reported prevalences. For instance, in the case of vascular BD, there is no consensus concerning the lesions that should be included in the clinical vascular characteristics of BD. Therefore, in Japan, patients with superficial thrombophlebitis are excluded from studies that examine the frequency of vascular involvement in BD, since this lesion is categorized as a skin manifestation according to the Japanese criteria.
4.2. Epidemiology of Vasculo-Behçet’s Disease and Coexistent Thrombophilic Factors
The term “vasculo-Behçet,” which is included in the specific types of BD according to the Japanese criteria of 2003, is used in order to identify that subcategory of the patients with BD who have a tendency to develop large vessel lesions, often with an additive and progressive course . The frequency of the vascular involvement among patients with BD ranges from 7.7 to 38% . The lesions involve mainly the veins (29% of BD cases) and less frequently the arteries (8–16% of BD cases) and include vasculitis, thrombosis, and aneurysms . Thrombosis represents the most usual manifestation, whereas the rupture of a pulmonary artery aneurysm consists the leading cause of death in vasculo-BD . Nevertheless, the precise worldwide prevalences of large vein thrombosis in BD could not be recorded in this literature review because the majority of the international studies examine the large vessel involvement as a whole and they do not use a common and clear case definition. In contrast, cardiac involvement with clinical implication is rather unusual, since the reported incidence range from 1% to 5% . Cardiac manifestations mainly include coronary aneurysms, pericarditis, endocarditis, myocarditis, and predominantly right-sided intracardiac thrombosis .
The pathophysiology of vascular thrombosis in BD is not well understood. It is generally accepted that the triggering feature in the thrombogenesis process is the vascular inflammation. However, the presence of large vessel thrombosis only in certain patients with BD implies that other additional factors are required for the development of a thrombotic event. La Regina et al. proposed that thrombogenesis in BD can be explained through the concept of Virchow’s triad of venous thrombosis (abnormal blood flow, abnormal vessel wall, and abnormal blood constituents) .
Several abnormal blood constituents have been implicated in the thrombogenesis in BD, with an emphasis given in certain procoagulant factors (factor V Leiden and prothrombin mutations, MTHFR polymorphisms, hyperhomocysteinemia, and antiphospholipid antibodies), but the results of the related researches are controversial (Table 6); Gül et al. found a positive association between FV Leiden and FII G20210A mutations with venous thrombosis in BD in Turkish patients [8, 9], whereas Silingardi et al. did not confirm these results in Italian patients . Karakus et al. reported that homozygosity of MTHFR C677T was significantly higher in BD patients than in healthy controls in Turkish patients , but Ricart et al. did not find such an association in patients from eastern Spain . Shahram et al. found significantly higher homocysteine levels in Iranian patients with BD and thrombosis , whereas Leiba et al. found homocysteine levels within normal range among Israeli patients with BD and thrombosis . Lastly, no association was found between anticardiolipin antibodies titres and vascular involvement in BD in Turkish patients by Tokay et al. , while statistically significant presence of aCL was reported in patients with BD in an Italian study by Hull et al. .
All these conflicting data get even more perplexing if we further examine the factor V Leiden (FVL) and prothrombin 20210A mutations. FVL is mainly encountered in Caucasians, with an average prevalence of 5%, and increases the risk of venous thrombosis 3- to 8-fold for heterozygous carriers and 50- to 80-fold for homozygous carriers . The Mediterranean region presents the highest prevalence of FVL in the world, while FVL is very rare or absent in non-Caucasian ethnic groups (Africans, South-East Asians, Chinese, Japanese, American Indians, Greenland Eskimos, and Aboriginals of Australia) . Likewise, FII 20210A mutation is met only in Caucasians with an average prevalence of 2-3% and increases the risk of venous thrombosis 3-fold . The highest prevalence of FII 20210A is encountered in the Southern Europe, in the Mediterranean region and in the Middle East [10, 37].
These epidemiologic data point out that the highest prevalences of both these inherited procoagulant factors are quite proportional to the highest epidemiologic prevalences of vasculo-BD, posing the question whether it is a coincidental coexistence or if there is a genetic association. Trying to answer this question, we examined random studies from literature review concerning the association between BD, FVL, and FII 20210A mutations. As we have noticed previously, FVL and FII 20210A are absent in Japan but prevalent in Turkey (% and 0.7–8.0%, resp.) and in Lebanon (% and 1.3–3.6%, resp.) [37, 38]. Assuming that the reports of Gül et al. [8, 9] indicate that FVL and FII 20210A mutations are genetically associated with BD and act synergistically with the tenderness of BD for thrombotic events, one could say that this is one reason that explains why the prevalence of vascular BD is lower in Japan (%)  and higher in Turkey (–26%) [29, 30] and in Lebanon (.8%) . Consequently, we should expect that this correlation applies in Italy and in Spain as well, where FVL and FII 20210A mutations are also frequently encountered; nevertheless, the studies from Silingardi et al.  and Espinosa et al.  did not find such a positive association between those thrombophilic mutations and BD in Italian and Spanish patients, respectively.
The main argumentations for the inconsistency of clinical, laboratory, and genetic findings in BD are the multifactorial interaction in the pathogenesis of the disease and the lack of case ascertainment, which in many patients result in an inappropriate diagnosis of a possible or an incomplete type of BD. Therefore, the worldwide studies may present a discrepancy in their results, on account of the fact that the experimental groups embody several subjects who actually do not suffer from BD. But then, in all these patients that present only certain features of BD, which is the nature of their actual disease? In a recently published paper, which was brought to our knowledge while writing this paper, Yazici et al. pose a similar questioning about the heterogeneity of BD and reach the conclusion that BD may actually represent more than one condition, underlining three more crucial parameters of BD . The first is that a Mendelian inheritance pattern does not seem to exist among the adult patients, whereas an autosomal recessive inheritance pattern has been suggested for the pediatric patients. The second is that a differing response of different clinical manifestations is encountered to one same drug. The third and most essential parameter is that two discrete symptom clusters can be recognized in BD at the present time, the acne and arthritis cluster and the vascular cluster, in which, the dural sinus thrombosis of neuro-BD and the superficial vein thrombosis, the deep vein thrombosis, and the pulmonary artery involvement of vasculo-BD all seem to be clustered in BD patients .
In the aforementioned three male patients, clinical presentation was dominated from the extended vena cava thrombosis. Their laboratory assessment revealed elevated inflammation markers, HLA-B*51 positivity, and several coexisting thrombophilic factors. Their thrombophilia was not enough to interpret their deteriorating large vessel thrombosis considering the failure of the anticoagulant therapy to prevent the expansion of the thrombosis. The exclusion of infectious and malignant causes suggested the possibility of an underlying autoimmune or autoinflammatory mechanism as a contributory factor in the lasting thrombogenesis process. Despite the absence of typical for BD ocular manifestations, the diagnostic assessment showed that this factor was BD in patient A and most probably in patient B as well, taking into consideration his direct response to the use of immunosuppressant agents. In patient C the lack of definite oral ulceration and a negative pathergy test or other clinical manifestations led us in a diagnostic stalemate.
The inconsistent and frequently contradictory features of BD, especially regarding vasculo-BD and coexistent thrombophilic factors that were presented previously, demonstrate the significant heterogeneity of this disease, leading us to the hypothesis that a lot of patients who are diagnosed as suffering from BD might not have the same disease but different overlapping clinical entities. We further assume that patients who present large vessel thrombosis, coexistent thrombophilic factors, and some BD features, for instance oral aphthosis and HLA-B*51 positivity, may actually suffer from a distinct nosological entity (Figure 5). In this clinical entity, the complex interaction among genetic loci could be explained with the phenomenon of epistasis. Epistasis, which is usually found in the human major histocompatibility complex genes, is a genetic term that was invented from William Bateson, who observed that, in some dihybrid crosses, not all possible phenotypic classes were observed and/or that some gene combinations resulted in novel phenotypes . The concept of synergistic epistasis could theoretically answer the question why certain patients with BD have a specific tendency for large vessel thrombosis. Besides, the existence of such a distinct nosological entity, which shares some features of the complex spectrum of BD, could explain why certain only international studies confirm a significant association between BD and specific thrombophilic factors, as well as why the frequency of vascular thrombosis in BD is lower in Japan, where some factors such as V Leiden and II G20210A mutations are absent.
Nevertheless, there is a range of limitations to this medical hypothesis that need to be acknowledged. The three cases presented in this paper comprise a negligible sample in order to reach definite conclusions. Furthermore, the relatively short duration of follow-up in patients B and C (less than a year) must be taken into consideration, since we cannot rule out the possibility that another known cause of thrombosis might be clinically evidenced in the forthcoming period. Besides, apart from the malignancies and the established immunological causes of thrombosis (systemic lupus erythematosus, inflammatory bowel disease, and BD), current evidence suggests that many other defined autoimmune disorders and immune-mediated diseases are closely associated with an increased risk of venous thromboembolism . Finally, even if the hypothesis of this paper was verifiable, it still seems that this theoretical model of venous thrombosis could not explain every single case of large vessel thrombosis in BD, given that in certain international studies it is demonstrated that thrombosis in BD can occur in spite of the absence of—at least all identified—thrombophilic factors [12, 14, 40].
Consequently, it is unquestionable that our hypothesis for the potential existence of a distinct nosological entity, which entails a genetic linkage between thrombophilia and a novel inflammatory condition that shares certain features of BD, cannot be documented only by the aforementioned cases and the analysis of the preexisting literature. Further studies are required, in order to determine if there is indeed a subgroup of patients with large vessel thrombosis, coexistent thrombophilia, and poor response to the anticoagulant therapy and at the same time they do present certain other common clinical manifestations and laboratory findings of BD. Moreover, the thrombotic phenomenon in these distinct overlapping clinical entities may emphasize the necessity of studying the coagulation physiology in combination with the immune response as it is studied in basic sciences . Recently, the term immunothrombosis has been suggested in order to describe a physiological form of thrombosis that supports innate immune defence in microvessels . The use of the term immunothrombosis could be suitable in order to describe thrombotic phenomena with immunological background in large vessels if any correlation is evidenced.
Epidemiologic data and varied genetic background suggest that BD might not constitute a single disease. Moreover, in a number of patients with large vessel thrombosis, coexistent thrombophilia, acute phase response, lack of malignancy or other known causes of thrombosis, and failure of the antithrombotic therapy alone to treat or at least prevent the expansion of the thrombosis, it seems that another inflammatory disorder is required in order to trigger the thrombogenesis process and therefore the basis for their treatment is immunosuppressant agents. In these cases that share various common features with BD, it is uncertain if the dilemma of the proper treatment, anticoagulant, and/or immunosuppressive treatment can be answered through the heterogeneous spectrum of BD. Distinguishing such patients as suffering from a distinct nosological entity is a hypothesis that can be proved only with further studies. On the contrary, if our hypothesis is incorrect and these patients do represent cases of BD with coincidental coexistent thrombophilia, taking into consideration the proneness of BD to thrombotic events and the highest concomitant epidemiologic prevalences of vasculo-BD and specific inherited procoagulant factors, we propose that all patients with BD who manifest thrombotic events should be assessed for thrombophilia testing, especially those living in Southern Europe, in the Mediterranean basin, and in the Middle East, so that their optimal management can be appropriately weighed.
A written informed consent was obtained from the patients for publication of this paper and accompanying images.
The opinions or assertions contained herein are the private views of the authors and are not to be construed as reflecting the Department or the Hospital.
Conflict of Interests
The authors declare that they have no conflict of interests.
- A. Gül, “Behçet's disease as an autoinflammatory disorder,” Current Drug Targets, vol. 4, no. 1, pp. 81–83, 2005.
- International Study Group for Behçet's Disease, “Criteria for diagnosis of Behçet's disease,” The Lancet, vol. 335, no. 8697, pp. 1078–1080, 1990.
- M. S. Kurokawa and N. Suzuki, “Behcet's disease,” Clinical and Experimental Medicine, vol. 4, no. 1, pp. 10–20, 2004.
- T. Hayashi and N. Mizuki, “Ocular manifestations in Behçet's disease,” Japan Medical Association Journal, vol. 49, no. 7-8, pp. 260–268, 2006.
- F. R. Rosendaal, “Venous thrombosis: the role of genes, environment, and behavior,” Hematology, vol. 2005, pp. 1–12, 2005.
- M. Biggioggero and P. L. Meroni, “The geoepidemiology of the antiphospholipid antibody syndrome,” Autoimmunity Reviews, vol. 9, no. 5, pp. A299–A304, 2010.
- M. M. Jadaon, “Epidemiology of activated protein C resistance and factor V leiden mutation in the mediterranean region,” Mediterranean Journal of Hematology and Infectious Diseases, vol. 3, no. 1, Article ID e2011037, 2011.
- A. Gül, U. Ozbek, C. Oztürk, M. Inanç, M. Koniçe, and T. Ozçelik, “Coagulation factor V gene mutation increases the risk of venous thrombosis in behçet's disease,” British Journal of Rheumatology, vol. 35, no. 11, pp. 1178–1180, 1996.
- A. Gül, A. B. Aslantas, T. Tekinay, M. Koniçe, and T. Ozçelik, “Procoagulant mutations and venous thrombosis in Behcet's disease,” Rheumatology, vol. 38, no. 12, pp. 1298–1299, 1999.
- M. Silingardi, C. Salvarani, L. Boiardi et al., “Factor V leiden and prothrombin gene G20210A mutations in Italian patients with Behçet's disease and deep vein thrombosis,” Arthritis Care and Research, vol. 51, no. 2, pp. 177–183, 2004.
- N. Karakus, S. Yigit, G. Kalkan, et al., “Association between the methylene tetrahydrofolate reductase gene C677T mutation and colchicine unresponsiveness in Behcet's disease,” Molecular Vision, vol. 18, pp. 1696–1700, 2012.
- J. M. Ricart, A. Vayá, J. Todolí, et al., “Thrombophilic risk factors and homocysteine levels in Behçet's disease in eastern Spain and their association with thrombotic events,” Journal of Thrombosis and Haemostasis, vol. 95, no. 4, pp. 618–624, 2006.
- F. Shahram, A. Faridar, M. G. Hamedani et al., “Plasma homocysteine level in patients with Behcet's disease with or without thrombosis,” Archives of Iranian Medicine, vol. 13, no. 6, pp. 476–481, 2010.
- M. Leiba, U. Seligsohn, Y. Sidi et al., “Thrombophilic factors are not the leading cause of thrombosis in Behçet's disease,” Annals of the Rheumatic Diseases, vol. 63, no. 11, pp. 1445–1449, 2004.
- S. Tokay, H. Direskeneli, S. Yurdakul, and T. Akoglu, “Anticardiolipin antibodies in Behçet's disease: a reassessment,” Rheumatology, vol. 40, no. 2, pp. 192–195, 2001.
- R. G. Hull, E. N. Harris, and A. E. Gharavi, “Anticardiolipin antibodies: occurrence in Behcet's syndrome,” Annals of the Rheumatic Diseases, vol. 43, no. 5, pp. 746–748, 1984.
- D.-K. Kim, S. N. C. Chang, D. Bang, E.-S. Lee, and S. Lee, “Clinical analysis of 40 cases of childhood-onset Behcet's disease,” Pediatric Dermatology, vol. 11, no. 2, pp. 95–101, 1994.
- M. B. Owlia and G. Mehrpoor, “Behcet's disease: new concepts in cardiovascular involvements and future direction for treatment,” ISRN Pharmacology, vol. 2012, Article ID 760484, 2012.
- R. Tunes and M. Santiago, “Behcet's syndrome: literature review,” Current Rheumatology Reviews, vol. 5, no. 1, pp. 64–82, 2009.
- F. A. Alnaimat and J. R. Lisse, “Behcet Disease,” Medscape, http://emedicine.medscape.com/article/329099-overview#a0199/.
- K. T. Calamia, F. C. Wilson, M. Icen, C. S. Crowson, S. E. Gabriel, and H. M. Kremers, “Epidemiology and clinical characteristics of behcet's disease in the us: a population-based study,” Arthritis Care and Research, vol. 61, no. 5, pp. 600–604, 2009.
- F. Parentin, L. Lepore, I. Rabach, and S. Pensiero, “Paediatric Behcet's disease presenting with recurrent papillitis and episcleritis: a case report,” Journal of Medical Case Reports, vol. 5, p. 81, 2011.
- D. H. Verity, G. R. Wallace, R. W. Vaughan, and M. R. Stanford, “Behçet's disease: from Hippocrates to the third millennium,” British Journal of Ophthalmology, vol. 87, no. 9, pp. 1175–1183, 2003.
- J. Karasneh, A. Gül, W. E. Ollier, A. J. Silman, and J. Worthington, “Whole-genome screening for susceptibility genes in multicase families with Behçet's disease,” Arthritis and Rheumatism, vol. 52, no. 6, pp. 1836–1842, 2005.
- F. Kaneko, A. Togashi, S. Saito et al., “Behçet's disease (Adamantiades-Behçet's disease),” Clinical and Developmental Immunology, vol. 2011, Article ID 681956, 7 pages, 2011.
- A. Al-Araji and D. P. Kidd, “Neuro-Behçet's disease: epidemiology, clinical characteristics, and management,” The Lancet Neurology, vol. 8, no. 2, pp. 192–204, 2009.
- E. C. Ebert, “Gastrointestinal manifestations of behçet's Disease,” Digestive Diseases and Sciences, vol. 54, no. 2, pp. 201–207, 2009.
- C. C. Zouboulis, “Epidemiology of Adamantiades-Behcet's disease,” Annales de Medecine Interne, vol. 150, no. 6, pp. 488–498, 1999.
- R. Sarica-Kucukoglu, A. Akdag-Kose, M. Kayabali et al., “Vascular involvement in Behçet's disease: a retrospective analysis of 2319 cases,” International Journal of Dermatology, vol. 45, no. 8, pp. 919–921, 2006.
- K. Sağdiç, Z. G. Ozer, D. Saba, M. Türe, and M. Cengiz, “Venous lesions in Behçet's disease,” European Journal of Vascular and Endovascular Surgery, vol. 11, no. 4, pp. 437–440, 1996.
- F. Davatchi, F. Shahram, C. Chams-Davatchi et al., “Behcet's disease in Iran: analysis of 6500 cases,” International Journal of Rheumatic Diseases, vol. 13, no. 4, pp. 367–373, 2010.
- H. Ideguchi, A. Suda, M. Takeno, A. Ueda, S. Ohno, and Y. Ishigatsubo, “Characteristics of vascular involvement in Behçet's disease in Japan: a retrospective cohort study,” Clinical and Experimental Rheumatology, vol. 29, no. 4, supplement 67, pp. S47–S53, 2011.
- P. G. Kaklamanis and K. T. Calamia, “Behçet's disease: an update on pathogenesis, diagnosis and management of vascular involvement,” Rheumatology Reports, vol. 2, no. 1, pp. 13–25, 2010.
- G. Cocco and A. Y. Gasparyan, “Behçet’s disease: an insight from a cardiologist’s point of view,” The Open Cardiovascular Medicine Journal, vol. 4, pp. 63–70, 2010.
- F. El Louali, A. Tamdy, A. Soufiani et al., “Cardiac thrombosis as a manifestation of Behçet syndrome,” Texas Heart Institute Journal, vol. 37, no. 5, pp. 568–571, 2010.
- M. la Regina, A. Y. Gasparyan, F. Orlandini, and D. Prisco, “Behçet's disease as a model of venous thrombosis,” Open Cardiovascular Medicine Journal, vol. 4, no. 1, pp. 71–77, 2010.
- M. M. Jadaon, “Epidemiology of prothrombin G20210A mutation in the mediterranean region,” Mediterranean Journal of Hematology and Infectious Diseases, vol. 3, no. 1, Article ID e2011054, 2011.
- N. Irani-Hakime, H. Tamim, R. Kreidy, and W. Y. Almawi, “The prevalence of factor V R506Q mutation-Leiden among apparently healthy Lebanese,” American Journal of Hematology, vol. 65, no. 1, pp. 45–49, 2000.
- A. Hamdan, W. Mansour, I. Uthman, A. F. Masri, F. Nasr, and T. Arayssi, “Behçet's disease in Lebanon: clinical profile, severity and two-decade comparison,” Clinical Rheumatology, vol. 25, no. 3, pp. 364–367, 2006.
- G. Espinosa, J. Font, D. Tàssies, et al., “Vascular involvement in Behçet's disease: relation with thrombophilic factors, coagulation activation, and thrombomodulin,” American Journal of Medicine, vol. 112, no. 1, pp. 37–43, 2002.
- H. Yazici, S. Ugurlu, and E. Seyahi, “Behçet syndrome: is it one condition?” Clinical Reviews in Allergy & Immunology, vol. 43, no. 3, pp. 275–280, 2012.
- P. C. Phillips, “Epistasis—the essential role of gene interactions in the structure and evolution of genetic systems,” Nature Reviews Genetics, vol. 9, no. 11, pp. 855–867, 2008.
- B. Zöller, X. Li, J. Sundquist, and K. Sundquist, “Autoimmune diseases and venous thromboembolism: a review of the literature,” American Journal of Cardiovascular Disease, vol. 2, no. 3, pp. 171–183, 2012.
- M. J. Hickey and P. Kubes, “Intravascular immunity: the host-pathogen encounter in blood vessels,” Nature Reviews Immunology, vol. 9, no. 5, pp. 364–375, 2009.
- B. Engelmann and S. Massberg, “Thrombosis as an intravascular effector of innate immunity,” Nature Reviews Immunology, vol. 13, no. 1, pp. 34–45, 2013.