International Scholarly Research Notices / 2012 / Article / Tab 1 / Review Article
Application of Different Analytical Techniques and Microbiological Assays for the Analysis of Macrolide Antibiotics from Pharmaceutical Dosage Forms and Biological Matrices Table 1 Summary of chromatographic conditions for the analysis of some macrolide antibiotics from bulk powder and different formulations in official monographs and literatures. Summary of chromatographic conditions for the analysis of some macrolide antibiotics from bulk powder and different formulations in official monographs and literatures.
(a) Analyte Stationary phase/column temp
(
∘
C
)
Mobile phase/flow rate Detection Reference Erythromycin lactobionate (i.v) and erythromycin tablet 4.6 mm
×
25 cm, 8–10 μ m, L21/70 3.5% K2 HPO4 (pH = 9): H2 O : 2-methyl propanol : acetonitrile (50 : 755 : 165 : 30)/1 mL/min UV-Vis at 215 nm [40 ] Erythromycin raw material 4.6 mm
×
25 cm, L21/70 0.2 M K2 HPO4 (pH = 9) : H2 O : tertiarybutyl alcohol : acetonitrile (50 : 755 : 165 : 30)/2 mL/min UV-Vis at 215 nm Clarithromycin (raw material and tablet) 4.6 mm
×
15 cm, L1 /50
CH
3
OH : .067 M KH2 PO4 (pH = 4) (65 : 35)/1 mL/min UV-Vis at 210 nm
[16 ] Clarithromycin (oral suspension) 4.6 mm
×
15 cm, L1 /50 CH3 OH : .067 M KH2 PO4 (pH = 4) (60 : 40)/1mL/min UV-Vis at 210 nm Azithromycin (raw material, capsule ) 4.6 mm
×
15 cm, L29 0.02 M KH2 PO4 : CH3 CN (pH = 11.0 ± 0.1) (2130 + 870)/1.5 mL/min Amperometrically Dirithromycin (raw and delayed release tablet) 4.6 mm
×
25 cm, 5
𝜇
m, L21/40 CH3 CN : Potassium phosphate buffer : CH3 OH (44 : 37 : 19)/2 mL/min UV-Vis at 205 nm Tylosin 4.6 mm
×
20 cm, 5
𝜇
m, L21 2 M sodium per chlorate (pH = 2.5 ± 0.1) : CH3 CN (60 : 40)/0.7 mL/min UV-Vis at 280 nm
(b)
Analyte(s) Stationary phase/column temp
(
∘
C
)
Mobile phase/flow rate (mL/min) Detection Reference Josamycin, kitasamycin, mirosamicin, spiramycin and tylosin 5C18 column (150
×
4.6 mm I.D.)/ambient Gradient A: 0.025 M phosphate buffer (pH 2.5) B: acetonitrile 287 for tylosin and 232 nm for the rest [28 ] Roxithromycin Lichrospher RP (125
×
4.6 mm, 5
𝜇
m)/40 0.05.m potassium dihydrogen phosphate : Acetonitrile, (30 : 70), l pH 7/2 mL/min Fluoresce
𝜆
ex 255 and
𝜆
em 315 [32 ] Azithromycin (in bulk powder, tablet, capsule and in vitro dissolution test) C18 (150
×
3.9 mm, 4
𝜇
m)/ambient 0.05 M ammonium acetate : acetonitrile : methanol : tetrahydrofuran (60 : 27 : 25 : 2.5)/ 1 mL/min Amperometrically [33 ] LiChroCART RP (125
×
4.6 mm, 5
𝜇
m)/ambient Buffer : acetonitrile : methanol (60 : 20 : 20), pH 8/1 mL/min UV-Vis at 215 nm [34 ] C18 (150 mm
×
2.1 mm, 5
𝜇
m)/ambient 20 mM ammonium acetate (pH 5.2) : acetonitrile : methanol (50 : 40 : 10)/0.2 mL/min Ms detection [35 ]