Analyte(s) Matrix Sample treatment HPLC column/temperature/mobile phase (v/v) Detection Reference Erythromycin, roxithromycin, azithromycin, and clarithromycin Human serum Liquid-liquid extraction, Diethyl ether C18 BDS (20
×
4.6 mm, 5 μ m) + C18 BDS (125
×
4.6 mm, 5 μ m)/0.05 M potassium dihydrogen phosphate which contains 0.5 mL triethylamine, pH 7.5 : acetonitile (60 : 40) Fluorescenceλ ex 255 and λ em 315 [31 ] Spiramycin, tilmicosin and tylosin Meat, liver, kidneys, raw milk, and eggs Solid-phase extraction, cation exchange cartridge C18 8
×
4 mm, 5 μ m + ODS RP 150
×
4 mm, 5 μ m A: phosphate buffer 0.05 mol/L pH 2.5/acetonitrile (80 : 20) B: acetinitrile MS/MS UV detection SPI = 320 nm; TIL + TYL = 287 nm [36 , 37 ] Erythromycin base Erythromycin estolate Erythromycin ethylsuccinate Urine plasma Liquid-liquid extraction, diethyl ether Nova-pack C18 /56 mM sodium acetate buffer : acetonitrile : methanol (56 : 50 : 4), pH 7.0 Amperometrically [41 ] Rosamicin Roxithromycin Urine Liquid-liquid extraction, acetonitrile Symmetry 300 C18 150 mm×2.1 mm, 3.5 μ m/25 mM phosphate buffer, pH 6.7 : water : acetonitrile (625 : 100 : 275) Amperometrically [42 ] Erythromycin Azithromycin Roxithromycin Clarithromycin Plasma Liquid-liquid extraction, tert-methyl-butylether YMC-Pack ODS-AP 250 mm×6.0 mm, 5 μ m/30°C/ACN : 50 mM phosphate buffer (pH 7.2) (43 : 57) Amperometrically [43 ] Erythromycin A Plasma Liquid-liquid extraction, diethyl ether μ Bondapack C18 300 mm×3.9 mm, 10μ m/ambient/acetonitrile : Methanol : 0.2 M sodium acetate (pH 6.7) (40 : 5 : 55)Coulometrically [44 ]