Volume 2012 (2012), Article ID 937265, 6 pages
Direct Electron Transfer of Cytochrome c on ZnO Nanoparticles Modified Carbon Paste Electrode
1Department of Biochemistry, Payam-e-Noor University, 7371719578 Tehran, Iran
2Department of Biology, Payam-e-Noor University, Yazd, Iran
Received 3 January 2012; Accepted 19 January 2012
Academic Editors: D. Bulone and M. P. Ponomarenko
Copyright © 2012 Masoud Negahdary et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The direct electrochemistry of cytochrome c (cyt c) immobilized on a modified carbon paste electrode (CPE) was described. The electrode was modified with ZnO nanoparticles. Direct electrochemistry of cytochrome c in this paste electrode was easily achieved, and a pair of well-defined quasireversible redox peaks of a heme Fe (III)/Fe(II) couple appeared with a formal potential (E0) of −0.303 V (versus SCE) in pH 7.0 phosphate buffer solution (PBS). The fabricated modified bioelectrode showed good electrocatalytic ability for reduction of H2O2. The preparation process of the proposed biosensor was convenient, and the resulting biosensor showed high sensitivity, low detection limit, and good stability.