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ISRN Pathology
Volume 2012 (2012), Article ID 691430, 7 pages
doi:10.5402/2012/691430
Research Article

Cyclin A2 Protein Overexpression Is Not Caused by Gene Amplification in Colon Cancer

Lise Aagaard Sørby,1 Kristin Jonsdottir,2 Klaus Beiske,3 Peter Blom,4 Ida Rashida Khan Bukholm,5,6,7 and Morten Bj. Jacobsen1,5,8

1Quality and Research Department, Ostfold Hospital Trust, Jernbanegaten 11, 2. Et, Postboks 16, 1603 Fredrikstad, Norway
2Institute of Clinical Epidemiology and Molecular Biology (EpiGen), Akershus University Hospital, 1478 Lørenskog, Norway
3Department of Pathology, Oslo University Hospital Rikshospitalet, 0027 Oslo, Norway
4Department of Pathology, Akershus University Hospital, 1478 Lørenskog, Norway
5Institute of Clinical Medicine, University of Oslo, 0316 Oslo, Norway
6Department of Surgery, Akershus University Hospital, 1478 Lørenskog, Norway
7Department of Health Promotion, Akershus University Hospital, 1478 Lørenskog, Norway
8Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, 1432 Ås, Norway

Received 8 June 2012; Accepted 19 July 2012

Academic Editors: C. E. Bacchi and M. Yamakawa

Copyright © 2012 Lise Aagaard Sørby et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. M. Malumbres and M. Barbacid, “Cell cycle, CDKs and cancer: a changing paradigm,” Nature Reviews Cancer, vol. 9, no. 3, pp. 153–166, 2009. View at Publisher · View at Google Scholar · View at Scopus
  2. J. K. Kim and J. A. Diehl, “Nuclear cyclin D1: an oncogenic driver in human cancer,” Journal of Cellular Physiology, vol. 220, no. 2, pp. 292–296, 2009. View at Publisher · View at Google Scholar · View at Scopus
  3. K. E. Knudsen, J. A. Diehl, C. A. Haiman, and E. S. Knudsen, “Cyclin D1: polymorphism, aberrant splicing and cancer risk,” Oncogene, vol. 25, no. 11, pp. 1620–1628, 2006. View at Publisher · View at Google Scholar · View at Scopus
  4. A. Husdal, G. Bukholm, and I. R. K. Bukholm, “The prognostic value and overexpression of cyclin A is correlated with gene amplification of both cyclin A and cyclin E in breast cancer patient,” Cellular Oncology, vol. 28, no. 3, pp. 107–116, 2006. View at Scopus
  5. I. R. K. Bukholm, G. Bukholm, and J. M. Nesland, “Over-expression of cyclin a is highly associated with early relapse and reduced survival in patients with primary breast carcinomas,” International Journal of Cancer, vol. 93, no. 2, pp. 283–287, 2001. View at Publisher · View at Google Scholar · View at Scopus
  6. R. Königsberg, O. Rögelsperger, W. Jäger et al., “Cell cycle dysregulation influences survival in high risk breast cancer patients,” Cancer Investigation, vol. 26, no. 7, pp. 734–740, 2008. View at Publisher · View at Google Scholar · View at Scopus
  7. R. Michalides, H. van Tinteren, A. Balkenende et al., “Cyclin A is a prognostic indicator in early stage breast cancer with and without tamoxifen treatment,” British Journal of Cancer, vol. 86, no. 3, pp. 402–408, 2002. View at Publisher · View at Google Scholar · View at Scopus
  8. J. J. M. Michels, F. Duigou, J. Marnay et al., “Flow cytometry and quantitative immunohistochemical study of cell cycle regulation proteins in invasive breast carcinoma: prognostic significance,” Cancer, vol. 97, no. 6, pp. 1376–1386, 2003. View at Publisher · View at Google Scholar · View at Scopus
  9. E. Baldini, A. Camerini, A. Sgambato et al., “Cyclin A and E2F1 overexpression correlate with reduced disease-free survival in node-negative breast cancer patients,” Anticancer Research, vol. 26, no. 6B, pp. 4415–4421, 2006. View at Scopus
  10. L. Li, K. Mu, G. Zhou, L. Lan, G. Auer, and A. Zetterberg, “Genomic instability and proliferative activity as risk factors for distant metastases in breast cancer,” British Journal of Cancer, vol. 99, no. 3, pp. 513–519, 2008. View at Publisher · View at Google Scholar · View at Scopus
  11. J. Bondi, A. Husdal, G. Bukholm, J. M. Nesland, A. Bakka, and I. R. K. Bukholm, “Expression and gene amplification of primary (A, B1, D1, D3, and E) and secondary (C and H) cyclins in colon adenocarcinomas and correlation with patient outcome,” Journal of Clinical Pathology, vol. 58, no. 5, pp. 509–514, 2005. View at Publisher · View at Google Scholar · View at Scopus
  12. J. Q. Li, H. Miki, F. Wu et al., “Cyclin a correlates with carcinogenesis and metastasis, and p27kip1 correlates with lymphatic invasion, in colorectal neoplasms,” Human Pathology, vol. 33, no. 10, pp. 1006–1015, 2002. View at Publisher · View at Google Scholar · View at Scopus
  13. K. Handa, M. Yamakawa, H. Takeda, S. Kimura, and T. Takahashi, “Expression of cell cycle markers in colorectal carcinoma: superiority of cyclin A as an indicator of poor prognosis,” International Journal of Cancer, vol. 84, no. 3, pp. 225–233, 1999.
  14. A. A. Bahnassy, A. R. N. Zekri, S. El-Houssini et al., “Cyclin A and cyclin D1 as significant prognostic markers in colorectal cancer patients,” BMC Gastroenterology, vol. 4, article 22, 2004. View at Publisher · View at Google Scholar · View at Scopus
  15. C. H. Yam, T. K. Fung, and R. Y. C. Poon, “Cyclin A in cell cycle control and cancer,” Cellular and Molecular Life Sciences, vol. 59, no. 8, pp. 1317–1326, 2002. View at Publisher · View at Google Scholar · View at Scopus
  16. M. F. Buckley, K. J. E. Sweeney, J. A. Hamilton et al., “Expression and amplification of cyclin genes in human breast cancer,” Oncogene, vol. 8, no. 8, pp. 2127–2133, 1993. View at Scopus
  17. U. Lehmann and H. Kreipe, “Real-time PCR analysis of DNA and RNA extracted from formalin-fixed and paraffin-embedded biopsies,” Methods, vol. 25, no. 4, pp. 409–418, 2001. View at Publisher · View at Google Scholar · View at Scopus
  18. P. Lichter, C. C. Tang, K. Call et al., “High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones,” Science, vol. 247, no. 4938, pp. 64–69, 1990. View at Scopus
  19. K. J. Livak and T. D. Schmittgen, “Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method,” Methods, vol. 25, no. 4, pp. 402–408, 2001. View at Publisher · View at Google Scholar · View at Scopus
  20. P. Platzer, M. B. Upender, K. Wilson et al., “Silence of chromosomal amplifications in colon cancer,” Cancer Research, vol. 62, no. 4, pp. 1134–1138, 2002. View at Scopus
  21. Z. Herceg and P. Hainaut, “Genetic and epigenetic alterations as biomarkers for cancer detection, diagnosis and prognosis,” Molecular Oncology, vol. 1, no. 1, pp. 26–41, 2007. View at Publisher · View at Google Scholar · View at Scopus
  22. R. Aamodt, K. Jonsdottir, S. N. Andersen, J. Bondi, G. Bukholm, and I. R. K. Bukholm, “Differences in protein expression and gene amplification of cyclins between colon and rectal adenocarcinomas,” Gastroenterology Research and Practice, vol. 2009, Article ID 285830, 9 pages, 2009. View at Publisher · View at Google Scholar · View at Scopus
  23. P. Szepetowski, D. Perucca-Lostanlen, and P. Gaudray, “Mapping genes according to their amplification status in tumor cells: contribution to the map of 11q13,” Genomics, vol. 16, no. 3, pp. 745–750, 1993. View at Publisher · View at Google Scholar · View at Scopus
  24. R. M. Seth, A. M. Burger, and H. J. Kahn, “Analysis of the HER2/neu gene amplification in microdissected breast cancer tumour samples,” Anticancer Research, vol. 26, no. 2A, pp. 927–931, 2006. View at Scopus
  25. X. Shi, J. Kleeff, Z. W. Zhu et al., “Gene-expression analysis of single cells-nested polymerase chain reaction after laser microdissection,” World Journal of Gastroenterology, vol. 9, no. 6, pp. 1337–1341, 2003. View at Scopus
  26. V. Bazan, G. La Rocca, S. Corsale et al., “Laser pressure catapulting (LPC): optimization LPC-system and genotyping of colorectal carcinomas,” Journal of Cellular Physiology, vol. 202, no. 2, pp. 503–509, 2005. View at Publisher · View at Google Scholar · View at Scopus