Table 1: Summary of the volumes of colloid and extended semen*, centrifuge tubes, and centrifuges used in these experiments (modified from [14]).

Species Tube size Volume of colloid (mL) Volume of extended semen* (mL) Name of technique Centrifuge Stallion/boar 12 mL 4.0 4.5 SLC-4.5 1 Stallion/boar 50 mL 15 15–18 SLC-15 1 Stallion/boar 100 mL 20 25 SLC-25 2 Stallion/boar 200 mL 60 60 SLC-60 3 Boar** 500 mL 150 150 SLC-150 1

*Extended to give a concentration of approximately 100 × 106 spermatozoa/mL. **this technique has not been attempted yet with stallion semen but is theoretically possible. Centrifuge 1: for example: Hereaus Multifuge, Kendro, Osterode, Germany; Centrifuge 2: for example: Sigma Laboratory Centrifuge, Osterode, Germany; Centrifuge 3: for example: Centra MP 4R, International Equipment Company, MA, USA.