Research Article
Isolation and Molecular Characterization of Potential Plant Growth Promoting Bacillus cereus GGBSTD1 and Pseudomonas spp. GGBSTD3 from Vermisources
Table 1
Predominant bacterial strains isolated from vermisources and their biochemical characteristics. T1-control—G. sepium + L. leucocephala + cow dung in (1 : 1 : 2) (worm free), T2—G. sepium + L. leucocephala + cow dung in (1 : 1 : 2) + E. eugeniae, T3—G. sepium + L. leucocephala + cow dung in (1 : 1 : 2) + E. fetida, T4-control (2)—C. auriculata + L. leucocephala + cow dung in (1 : 1 : 2) (worm free), T5—C. auriculata + L. leucocephala + cow dung in (1 : 1 : 2) + E. eugeniae, T6—C. auriculata + L. leucocephala + cow dung in (1 : 1 : 2) + E. fetida.
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S. number |
Sample source | Biochemical characteristicsc |
Name of the strain | | Gram’s reactiona | Cell shape | Motilityb | Indole test | Methyl Red test | Voges Proskauer test | Citrate Utilization test | Catalase test | Urease test | Gelatin hydrolysis test | Nitrate reductase test | Starch hydrolysis test | Casein hydrolysis test | Glucose utilization test |
| | 1. | Vermicompost (T2) | + | Cocci | − | − | + | − | − | + | − | + | + | − | − | − | Staphylococcus spp. | | 2. | Vermicompost (T3) | + | Rod | + | − | − | − | − | + | − | − | + | − | − | + | Corynebacterium spp. | | 3. | Vermicompost (T5) | + | Rod | − | + | + | + | + | + | − | + | + | + | − | + | Bacillus spp. | | 4. | Vermicompost (T6) | + | Cocci | − | + | + | − | − | + | − | + | − | − | − | + | Micrococcus spp. | | 5. | Vermicast (T3) | + | Cocci | − | + | + | − | − | − | + | + | + | + | − | − | Klebsiella spp. | | 6. | Vermicast (T5) | + | Rod | − | + | − | − | + | − | + | − | + | − | − | + | Streptococcus spp. | | 7. | Vermicast (T6) | + | Cocci | − | + | − | + | + | − | − | + | + | − | − | + | Enterobacter spp. | | 8. | Earthworm hind gut (T2) | + | Cocci | − | − | + | + | − | + | − | − | − | + | + | + | Tricoccocus spp. | | 9. | Earthworm hind gut (T3) | + | Cocci | − | − | + | − | + | + | + | − | + | − | − | − | Yersinia spp. | | 10. | Earthworm hind gut (T5) | + | Cocci | − | − | − | − | − | + | + | + | + | − | − | − | Micrococcus spp. | | 11. | Vermicompost (T1) | − | Rod | − | + | + | − | − | − | − | + | + | − | − | + | Escherichia spp. | | 12. | Vermicompost (T2) | − | Rod | − | − | + | − | − | − | + | + | + | − | − | + | Proteus spp. | | 13. | Vermicompost (T4) | − | Rod | − | − | − | + | − | + | − | − | + | + | + | + | Paracoccus spp. | | 14. | Vermicompost (T5) | − | Rod | + | + | − | − | − | + | + | + | + | − | − | − | Pseudomonas spp. | | 15. | Vermicast (T2) | − | Rod | + | − | + | − | + | + | + | − | + | − | − | − | Citrobacter spp. | | 16. | Vermicast (T3) | − | Cocci | − | + | + | − | − | + | − | + | − | − | − | − | Erwinia spp. | | 17. | Vermicast (T5) | − | Rod | + | + | + | + | + | + | − | + | − | − | − | − | Alcaligenes spp. | | 18. | Vermicast (T6) | − | Cocci | − | − | + | + | + | + | − | + | + | − | − | − | Serratia spp. | | 19. | Earthworm mid gut (T2) | − | Rod | + | − | − | − | + | − | − | − | − | − | − | − | Pediococcus spp. |
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Gram’s staining: (+) positive, (−) negative.
bMotility: (+) motile, (−) nonmotile.
cBiochemical characteristics: (+) positive, (−) negative.
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