Isolation and Molecular Characterization of Potential Plant Growth Promoting Bacillus cereus GGBSTD1 and Pseudomonas spp. GGBSTD3 from Vermisources
Table 3
Shoot length and Root length of Vigna unguiculata grown on vermiculite supplemented with Bacillus cereus, with Pseudomonas spp., and with their consortium (1 : 1) at five different concentrations (1, 2, 3, 4, and 5 mL) on the 5th, the 10th, and the 15th day and in the control.
Experimentsa
Quantity of bacterial cultures added
Shoot length (cm)b
Root length (cm)b
5th day
10th day
15th day
5th day
10th day
15th day
Exp0
1 mL
2.97 (ab)
3.97 (a)
4.97 (ab)
1.97 (de)
2.83 (ab)
3.97 (bcd)
2 mL
3.03 (bcd)
4.03 (ab)
5.00 (ab)
2.00 (abc)
2.87 (de)
4.00 (a)
3 mL
3.10 (abc)
4.07 (a)
5.03 (ab)
2.03 (de)
2.90 (abc)
4.03 (ab)
4 mL
3.10 (bcd)
4.10 (bcd)
5.07 (bcd)
2.07 (a)
2.97 (de)
4.10 (abc)
5 mL
3.13 (a)
4.13 (ab)
5.10 (ab)
2.10 (bcd)
3.00 (ab)
4.13 (de)
Exp1
1 mL
3.40 (ab)
4.40 (abc)
5.40 (ab)
2.33 (abc)
3.27 (bcd)
4.33 (f)
2 mL
4.50 (ab)
5.40 (abc)
6.47 (ab)
3.33 (ab)
4.30 (a)
5.40 (abc)
3 mL
5.50 (de)
6.30 (ab)
7.50 (a)
4.40 (de)
5.50 (abc)
6.20 (a)
4 mL
6.53 (ab)
7.30 (a)
8.33 (ab)
5.50 (bcd)
6.53 (de)
7.50 (abc)
5 mL
7.40 (ab)
8.40 (abc)
9.23 (f)
6.40 (abc)
7.53 (ab)
8.27 (ab)
Exp2
1 mL
2.77 (bcd)
3.57 (f)
4.40 (de)
1.60 (de)
2.17 (abc)
3.50 (f)
2 mL
3.43 (abc)
4.47 (ab)
5.53 (ab)
2.53 (abc)
3.40 (bcd)
4.27 (bcd)
3 mL
4.37 (de)
5.43 (abc)
6.33 (abc)
3.40 (ab)
4.43 (f)
5.33 (ab)
4 mL
5.47 (bcd)
6.47 (de)
7.33 (a)
4.20 (bcd)
5.50 (ab)
6.33 (ab)
5 mL
6.40 (f)
7.33 (bcd)
8.33 (bcd)
5.33 (ab)
6.30 (ab)
7.30 (bcd)
Exp3
1 mL
5.30 (bcd)
6.47 (ab)
7.30 (ab)
4.33 (de)
5.50 (abc)
6.20 (ab)
2 mL
6.43 (ab)
7.40 (bcd)
8.27 (f)
5.47 (a)
6.30 (de)
7.33 (ab)
3 mL
7.37 (bcd)
8.40 (bcd)
9.27 (ab)
6.40 (ab)
7.40 (bcd)
8.40 (bcd)
4 mL
8.47 (abc)
9.30 (a)
10.43 (abc)
7.40 (f)
8.33 (a)
9.37 (abc)
5 mL
9.57 (ab)
10.30 (f)
11.47 (bcd)
8.33 (abc)
9.40 (ab)
10.27 (bcd)
Exp0: nutrient solution (control), Exp1: Bacillus cereus, Exp2: Pseudomonas spp., Exp3: consortium of Bacillus cereus + Pseudomonas spp. (1 : 1).
bMean of two repeated experiments (30 plants). Different letters within parenthesis indicate significant difference between treatments for each growth parameter using Duncan’s multiple range test .