Research Article

Isolation and Molecular Characterization of Potential Plant Growth Promoting Bacillus cereus GGBSTD1 and Pseudomonas spp. GGBSTD3 from Vermisources

Table 4

Leaf area of Vigna unguiculata grown on vermiculite supplemented with Bacillus cereus, with Pseudomonas spp., and with their consortium (1 : 1) at five different concentrations (1, 2, 3, 4, and 5 mL) on the 5th, the 10th, and the 15th days and in the control.

ExperimentsaQuantity
of bacterial cultures added
Leaf area (cm2)b
5th day10th day15th day

Exp01 mL0.27 (ab)0.94 (abc)4.07 (b)
2 mL0.35 (ab)1.02 (ab)4.19 (abc)
3 mL0.40 (abc)1.25 (ab)4.71 (bcd)
4 mL0.48 (ab)1.38 (abc)5.10 (ab)
5 mL0.54 (abc)1.61 (b)5.38 (b)

Exp11 mL0.18 (bcd)1.23 (b)6.01 (abc)
2 mL0.25 (ab)1.38 (abc)6.32 (ab)
3 mL0.33 (abc)1.50 (ab)6.84 (b)
4 mL0.38 (ab)1.68 (ab)6.86 (abc)
5 mL0.44 (abc)1.91 (bcd)7.33 (ab)

Exp21 mL0.11 (b)1.07 (ab)5.34 (b)
2 mL0.14 (ab)1.19 (ab)5.59 (ab)
3 mL0.17 (abc)1.27 (ab)5.79 (ab)
4 mL0.21 (ab)1.53 (abc)6.31 (abc)
5 mL0.26 (ab)1.66 (b)6.67 (b)

Exp31 mL0.26 (bcd)2.97 (ab)7.53 (bcd)
2 mL0.33 (b)3.25 (ab)7.87 (ab)
3 mL0.40 (ab)3.50 (bcd)8.11 (bcd)
4 mL0.44 (b)3.84 (b)8.61 (b)
5mL0.62 (ab)4.00 (bcd)9.05 (ab)

Exp0: nutrient solution (control), Exp1: Bacillus cereus, Exp2: Pseudomonas spp., Exp3: consortium of Bacillus  cereus + Pseudomonas spp. (1 : 1).
bMean of two repeated experiments (30 plants). Different letters within parenthesis indicate significant difference between treatments for each growth parameter using Duncan’s multiple range test .