Isolation and Molecular Characterization of Potential Plant Growth Promoting Bacillus cereus GGBSTD1 and Pseudomonas spp. GGBSTD3 from Vermisources
Table 5
Chlorophyll content of leaves of Vigna unguiculata grown on vermiculite supplemented with Bacillus cereus, with Pseudomonas spp., and with their consortium (1 : 1) at five different concentrations (1, 2, 3, 4, and 5 mL) on the 5th day, the 10th day, and the 15th day and in the control.
Experimentsa
Quantity of bacterial Cultures added
Chlorophyll content (mg/g of fresh leaf)b
5th day
10th day
15th day
Chlorophyll a
Chlorophyll b
Total chlorophyll
Chlorophyll a
Chlorophyll b
Total chlorophyll
Chlorophyll a
Chlorophyll b
Total chlorophyll
Exp0
1 mL
0.21 (ab)
2.85 (abc)
3.07 (a)
0.20 (abc)
4.81 (a)
5.01 (ab)
0.15 (bcd)
6.12 (abc)
6.27 (bcd)
2 mL
0.22 (abc)
2.93 (ab)
3.15 (abc)
0.21 (ab)
4.89 (ab)
5.10 (bcd)
0.12 (abc)
6.18 (ab)
6.30 (ab)
3 mL
0.23 (abc)
3.01 (ab)
3.15 (abc)
0.22 (bcd)
4.97 (bcd)
5.18 (a)
0.14 (a)
6.33 (cde)
6.47 (bcd)
4 mL
0.24 (ab)
3.08 (f)
3.32 (a)
0.22 (a)
5.04 (b)
5.27 (abc)
0.15 (bcd)
6.41 (cde)
6.56 (a)
5 mL
0.25 (f)
3.16 (abc)
3.41 (bcd)
0.19 (ab)
5.11 (ab)
5.30 (abc)
0.11 (cde)
6.45 (ab)
6.50 (abc)
Exp1
1 mL
0.35 (b)
4.00 (cde)
4.35 (abc)
0.11 (b)
5.81 (cde)
5.93 (cde)
0.10 (abc)
7.04 (abc)
7.15 (f)
2 mL
0.26 (abc)
4.62 (bcd)
4.88 (f)
0.11 (abc)
6.47 (b)
6.58 (abc)
0.06 (ab)
7.79 (bcd)
7.85 (a)
3 mL
0.21 (bcd)
5.26 (abc)
5.47 (ab)
0.08 (bcd)
7.18 (ab)
7.26 (bcd)
0.06 (f)
8.47 (f)
8.53 (ab)
4 mL
0.08 (f)
5.87 (cde)
5.95 (b)
0.11 (bcd)
7.78 (abc)
7.89 (abc)
0.04 (bcd)
9.33 (b)
9.37 (bcd)
5 mL
0.26 (abc)
6.41 (bcd)
6.67 (abc)
0.25 (cde)
8.34 (cde)
8.59 (f)
0.07 (abc)
9.76 (cde)
9.83 (abc)
Exp2
1 mL
0.30 (abc)
3.54 (ab)
3.84 (b)
0.12 (a)
5.45 (abc)
5.56 (ab)
0.07 (bcd)
6.81 (abc)
6.88 (bcd)
2 mL
0.29 (bcd)
4.20 (f)
4.49 (abc)
0.11 (bcd)
6.10 (ab)
6.21 (abc)
0.07 (abc)
7.44 (f)
7.52 (bcd)
3 mL
0.22 (ab)
5.04 (abc)
5.27 (bcd)
0.15 (a)
6.77 (cde)
6.92 (bcd)
0.06 (bcd)
8.12 (ab)
8.17 (bcd)
4 mL
0.10 (f)
5.66 (a)
5.76 (cde)
0.18 (abc)
7.44 (f)
7.62 (ab)
0.05 (ab)
8.85 (a)
8.90 (abc)
5 mL
0.06 (bcd)
6.22 (abc)
6.28 (abc)
0.18 (b)
8.10 (abc)
8.28 (a)
0.04 (cde)
9.48 (a)
9.52 (f)
Exp3
1 mL
0.27 (ab)
4.70 (bcd)
4.96 (cde)
0.09 (ab)
6.68 (b)
6.77 (cde)
0.10 (a)
8.14 (ab)
8.25 (a)
2 mL
0.20 (ab)
5.48 (a)
5.68 (f)
0.03 (bcd)
7.46 (bcd)
7.49 (ab)
0.09 (ab)
8.87 (f)
8.97 (ab)
3 mL
0.05 (bcd)
5.84 (abc)
5.89 (ab)
0.08 (bcd)
8.04 (abc)
8.12 (cde)
0.10 (ab)
9.53 (abc)
9.63 (bcd)
4 mL
0.23 (f)
6.63 (ab)
6.86 (cde)
0.07 (ab)
8.70 (b)
8.77 (f)
0.10 (abc)
10.19 (bcd)
10.29 (f)
5 mL
0.22 (ab)
7.44 (cde)
7.66 (a)
0.09 (a)
9.32 (abc)
9.40 (ab)
0.10 (a)
10.85 (ab)
10.95 (abc)
Exp0: nutrient solution (control), Exp1: Bacillus cereus, Exp2: Pseudomonas spp., Exp3: consortium of Bacillus cereus + Pseudomonas spp. (1 : 1).
bMean of two repeated experiments (30 plants). Different letters within parenthesis indicate significant difference between treatments for each growth parameter using Duncan’s multiple range test .
