Recurrence Quantification Analysis of Spontaneous Electrophysiological Activity during Development: Characterization of In Vitro Neuronal Networks Cultured on Multi Electrode Array Chips

Table 1

MBR [burst/min]	DIV 10	13	14	15	16	17	20	21	22	23	28	29	30	42
mean	0.0100	0.0145	0.0103	0.0066	0.0122	0.0112	0.0115	0.0369	0.1184	0.1269	0.1083	0.0574	0.0358	0.0126
std	0.0097	0.0117	0.0042	0.0017	0.0040	0.0078	0.0061	0.0197	0.0946	0.0876	0.0568	0.0366	0.0213	0.0055
errstd	0.0069	0.0083	0.0021	0.0010	0.0023	0.0045	0.0022	0.0070	0.0315	0.0292	0.0201	0.0129	0.0075	0.0019
cv	0.9751	0.8094	0.4047	0.2554	0.3266	0.6953	0.5315	0.5335	0.7988	0.6904	0.5247	0.6375	0.5959	0.4373
Mean Burst Number [burst]	DIV 10	13	14	15	16	17	20	21	22	23	28	29	30	42
mean	6.50	9.50	6.75	4.33	8.00	7.33	7.63	24.38	78.11	83.78	71.50	37.88	23.63	8.25
std	6.36	7.78	2.75	1.15	2.65	5.13	4.07	13.05	62.43	57.83	37.54	24.11	14.07	3.65
errstd	4.50	5.50	1.38	0.67	1.53	2.96	1.44	4.61	20.81	19.28	13.27	8.52	4.97	1.29
cv	0.98	0.82	0.41	0.27	0.33	0.70	0.53	0.54	0.80	0.69	0.53	0.64	0.60	0.44
Burst Amplitude [spike]	DIV 10	13	14	15	16	17	20	21	22	23	28	29	30	42
mean	12.341	14.508	15.444	17.178	16.520	15.624	19.990	24.541	20.501	22.719	25.051	29.409	28.492	22.730
std	2.603	2.487	4.258	4.903	2.379	2.530	7.710	8.143	5.906	7.670	7.536	10.497	9.343	6.042
errstd	1.841	1.758	2.129	2.831	1.374	1.460	2.726	2.879	1.969	2.557	2.664	3.711	3.303	2.136
cv	0.211	0.171	0.276	0.285	0.144	0.162	0.386	0.332	0.288	0.338	0.301	0.357	0.328	0.266
Burst Duration [ms]	DIV 10	13	14	15	16	17	20	21	22	23	28	29	30	42
mean	172.123	222.367	172.589	149.433	141.071	133.695	104.138	144.781	109.939	117.365	133.208	188.610	168.383	155.034
std	55.611	6.741	58.799	29.039	38.274	30.332	40.088	31.857	25.810	25.205	22.030	42.591	40.396	29.909
errstd	39.323	4.767	29.400	16.766	22.098	17.512	14.173	11.263	8.603	8.402	7.789	15.058	14.282	10.574
cv	0.323	0.030	0.341	0.194	0.271	0.227	0.385	0.220	0.235	0.215	0.165	0.226	0.240	0.193
Weighted Burst Duration [ms]	DIV 10	13	14	15	16	17	20	21	22	23	28	29	30	42
mean	344.2455	3335.5	862.9438	747.1667	987.4975	802.1718	833.1023	2895.61	4947.242	5868.258	8525.282	4149.423	3536.049	930.2023
std	111.2215	101.1163	293.9954	145.1942	267.9214	181.9895	320.7046	637.1416	1161.467	1260.242	1409.888	936.9954	848.3061	179.4547
errstd	78.64545	71.5	146.9977	83.82789	154.6845	105.0717	113.3862	225.2636	387.1557	420.0806	498.4709	331.2779	299.9215	63.44683
cv	344.2455	3335.5	862.9438	747.1667	987.4975	802.1718	833.1023	2895.61	4947.242	5868.258	8525.282	4149.423	3536.049	930.2023
Mean Inter Burst Interval [ms]	DIV 10	13	14	15	16	17	20	21	22	23	28	29	30	42
mean	7737.76	12622.38	7295.26	8866.79	22472.71	13806.17	30529.00	19838.58	6854.69	11606.48	7557.16	22677.82	21249.86	8451.73
std	10942.84	17850.74	6365.85	7678.88	16692.84	14362.34	18160.49	9445.50	6587.63	9813.10	4118.10	8995.54	10881.04	7274.24
errstd	7737.76	12622.38	3182.92	4433.40	9637.62	8292.10	6420.70	3339.49	2195.88	3271.03	1455.97	3180.41	3847.03	2571.83
cv	1.41	1.41	0.87	0.87	0.74	1.04	0.59	0.48	0.96	0.85	0.54	0.40	0.51	0.86
Weighted IBI [ms]	DIV 10	13	14	15	16	17	20	21	22	23	28	29	30	42
mean	3868.88	841.4921	1459.052	1773.359	3210.387	2301.029	3816.1248	991.9288	152.3263	232.1296	118.0806	1030.81	1011.898	1408.621
std	5471.422	1190.05	1273.17	1535.776	2384.691	2393.723	2270.0611	472.2748	146.3917	196.2619	64.34534	408.8884	518.1445	1212.373
errstd	3868.88	841.4921	636.5849	886.6809	1376.802	1382.017	802.58782	166.9744	48.79723	65.42064	22.74951	144.5639	183.1918	428.6386
cv	3868.88	841.4921	1459.052	1773.359	3210.387	2301.029	3816.1248	991.9288	152.3263	232.1296	118.0806	1030.81	1011.898	1408.621