The Role of TWIST in Angiogenesis and Cell Migration in Giant Cell Tumor of Bone
Figure 4
TWIST promotes migration in GCT cells. (a) Wound-healing assay: GCT untransfected (UT) and TWIST transfected (GCTT) cells were scraped and measured after 24 h. The “wounded” distances were examined at the indicated time points under 100x magnification. (b) Cell migration was calculated in percentage compared to the control UT. (c) Cell migration assay: migration assays were performed using GCT cells transfected with a TWIST overexpression vector (GCTT) and the empty vector (EV) control cells in transwell plates. Shown are representative pictures of the cell migration assay after staining the cells from the underside of the insert. (d) Cell migration based on cell density on the undersurface of the insert is shown graphically.
