Research Article

Sodium Butyrate Plus EGF and PDGF-BB Aids Cutaneous Wound Healing in Diabetic Mice

Table 2

Time-line for STZ treatment, wounding, topical application of growth factor(s) and Na-Bu, and tissue harvest.

Day 0D1 to D5D6 to D15D16
W1
D17
W2
D18
W3
D19
W4
D20
W5
D21
W6
D22
W7
D23
W8
D24
W9
D25
W10
D26
W11
D27
W12
D28
W13
D29
W14

20 h STRSTZTTTTTTTTTTTTTTTTTTTTTTTTTTTT

G0G1G7
G15
G16G22G29

TH1TH7TH14

Day 0 is the beginning of the experiments. The animals were 6 weeks old and they were starved (STR) for 20 h prior to the induction of diabetes. STZ, daily injection of streptozotocin at a concentration of 40 mg/Kg body weight for consecutive 5 days (D1 to D5). Ten days (D6 to D15) after the last STZ injection, the wounds (W) were created and the day of the wounding is taken as W1; TT, various topical treatments as given in Table 4. G, measurement of glucose levels on the days after beginning the experiment; TH, days when the tissues were harvested for western blotting.