Merging Mixture Components for Cell Population Identification in Flow Cytometry
Figure 5
Detecting failed cluster merging. (a) Distribution of the entropy (normalized for the number of events and clusters) of the flowMerge solution for forward versus side scatter (left) and fluorescence channels (right) across 137 samples. (b) The relationship between the normalized entropy and the number of clusters in the flowMerge solution for forward scatter versus side scatter (left) and fluorescence channels (right). (c) Example of flowMerge solutions with unusually high normalized entropy from the right tail of the distribution for forward versus side scatter (left) and fluorescence (right). (d) A plot of the normalized entropy versus samples grouped by antibody labels identifies antibody combinations that are problematic for automated gating with the automated merging algorithm.
