Fluorescence Intensity Normalisation: Correcting for Time Effects in Large-Scale Flow Cytometric Analysis
Figure 5
(a) Repeatability for the CD4 memory T cell population CD25-APC MFI normalised using a multiplicative correction factor estimated by a regression analysis on the set of 149 samples with identical T1D susceptible genotype. (b) Repeatability for the CD4 memory T cell population CD25-APC MFI divided by the CD25-APC MFI in the full CD4 T cell gate for the same sample/analysed tube.