Automatic Clustering of Flow Cytometry Data with Density-Based Merging

Figure 1

Comparison of manual and DBM gating in the scatter dimensions—singlet gates are shown as determined by the researcher (top) and DBM (bottom, colored plot frames) for neonatal mouse spleen cells. The subset is further gated using the researcher's live/dead gate and displayed in context of the next gating decision by the researcher. Note that the results of the DBM clustering are displayed with same software that was used for the manual gating (FlowJo). This was done to facilitate the comparison and because a suitable display system for publication has not yet been developed. Thus in the bottom left plot, color is used to code the clusters found by DBM.