Bridging the Divide between Manual Gating and Bioinformatics with the Bioconductor Package flowFlowJo
Figure 2
Gates for the monocyte population as produced by the three gating procedures applied to two of the 96 whole blood samples. The distribution of the cells is indicated by the blue shading with darker blue corresponding to regions containing higher numbers of cells. Regions where a probability density function fit to the data was calculated to have significant curvature are indicated in black, except where they lie within the manual gate and are colored red. Gating methods I, II, and III are shown in green, yellow, and red, respectively. The regions were colored in order of largest to smallest for visual display because the gates overlap with each other.
