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Advances in Bioinformatics
Volume 2011 (2011), Article ID 184731, 11 pages
http://dx.doi.org/10.1155/2011/184731
Research Article

Data-Driven Compensation for Flow Cytometry of Solid Tissues

1Department of Pathology, Atrium Medical Centre, P.O. Box 4446, 6401 CX Heerlen, The Netherlands
2TiCC, Tilburg University, Warandelaan 2, 5037 AB Tilburg, The Netherlands

Received 3 February 2011; Revised 20 May 2011; Accepted 8 June 2011

Academic Editor: Shandar Ahmad

Copyright © 2011 Nickolaas Maria van Rodijnen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Propidium Iodide is a fluorochrome that is used to measure the DNA content of individual cells, taken from solid tissues, with a flow cytometer. Compensation for spectral cross-over of this fluorochrome still leads to compensation results that are depending on operator experience. We present a data-driven compensation (DDC) algorithm that is designed to automatically compensate combined DNA phenotype flow cytometry acquisitions. The generated compensation values of the DDC algorithm are validated by comparison with manually determined compensation values. The results show that (1) compensation of two-color flow cytometry leads to comparable results using either manual compensation or the DDC method; (2) DDC can calculate sample-specific compensation trace lines; (3) the effects of two different approaches to calculate compensation values can be visualized within one sample. We conclude that the DDC algorithm contributes to the standardization of compensation for spectral cross-over in flow cytometry of solid tissues.