Flux Analysis of the Trypanosoma brucei Glycolysis Based on a Multiobjective-Criteria Bioinformatic Approach
Figure 4
The flux prediction for the well-known BSF model in aerobiosis (a) and anaerobiosis (b). All the considered pathways are located in 3 compartments: mitochondrion, glycosomes, and cytosol. Circles represent metabolites, squares depict reactions, and blue squares are transporters. The stoichiometry is 1 by default, except for H+ and H2O (the stoichiometries of H+, H2O and O2 were multiplied by 2, resp. 4, 2 and 1). The quantity of input metabolites for the initiation stage of Metaboflux is colored in orange, and the final amount are then colored in purple. The flux percentage at branching points of the metabolic network is given in red for the DHAP node (step 5 versus step 11) and in blue for the Gly3P node (transport_1 versus step 15). The percentage values at branching points have to be considered as a subpart of the 100% of flux given for the branching point where two enzymes use the same metabolite; for instance, glycerol 3-phosphate dehydrogenase (step 11) and triose phosphate isomerase (step 5) use DHAP as substrate.
