Alternate Phosphorylation/O-GlcNAc Modification on Human Insulin IRSs: A Road towards Impaired Insulin Signaling in Alzheimer and Diabetes

Figure 7

Graphical representation of the potential Ser and Thr residues for O-β -GlcNAc as well as yin yang sites in human IRS-2. (a) Predicted potential sites for O-β -GlcNAc modification of Ser and Thr. Green vertical line shows O-β-GlcNAc modification potential of Ser/Thr residues, while the light blue wavy line indicates the threshold for modification potential. YinOYang 1.2 web based program generated this graph. A total number of 64 potential Ser/Thr residues showed O-β-GlcNAc modification potential including Ser-3, 162, 304, 308, 311, 357, 384, 400, 428, 438, 444, 449, 482, 483, 485, 489, 523, 560, 591, 592, 606, 615, 619, 620, 665, 679, 684, 704, 714, 731, 736, 848, 941, 944, 945, 946, 952, 953, 956, 966, 967, 984, 988, 1012, 1022, 1024, 1026, 1027, 1048, 1103, 1149, 1162, and 1203 and Thr-344, 443, 713, 719, 777, 844, 965, 1157, 1159, 1202, and 1319. (b) Predicted yin yang residues for human IRS-1. A total of 41 Ser/Thr residues showed potential for possible yin yang sites for phosphorylation and O-glycosylation interplay.

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