Prediction of Epitope-Based Peptides for the Utility of Vaccine Development from Fusion and Glycoprotein of Nipah Virus Using In Silico Approach

Table 1

Conserved sequences from the glycoprotein G and fusion protein F of Nipah virus.

(a)

F-protein	Position of the selected sequences	Prediction of transmembrane helix using TMHMM server	VaxiJen value
ISECSVGILHYEKLSKIGLVKG	19–41	Outside	0.67
TRKYKIKSNPLTKDIVIKMI	43–61	Inside	0.17
NVSNMSQCTEIYKNNTHDLVGDVRLAGVIMAGSVMENYKTRLNGILT; PIKGALGVAIGIATAAQITAGVALYEAMKNADNINKLKSSIESTNEAVV; KLQETAEKTVYVLTALQDYINTNLVPTIDKISCKQTELSLDLALSKY	64–112	Outside	0.44
	113–135	TM helix
	136–206	Inside
SDLLFVFGPNLQDPVSNSMTIQAISQAFGGNYETLLRTLGYA	208–249	Outside	0.21
FDDLLESDSITGQIIYVDLS	253–272	Outside	0.39
YYIIVRVYFPILTEIQQAYIQELLPVSFNNDNSEWISIVPNFILVRNTLISNI; EIGFCLITKRSVICNQDYATP	274–347	Outside	0.72
TNNMRECLTGSTEKCPRELVVSSHVPRFALSNGVLFANCISVTCQCQT; TGRAISQSGEQTLLMIDNTTCPTAVLGNVIISLGKYLGSVNYNSEGIAIG; PPVFTDKVDISSQ	349–459	Outside	0.41
SSMNQSLQQSKDYIKEAQRLLDTVNPSLISMLSMIILYVLSIASLCIGLIT; FISFIIVEKKRNTYSRLEDRRVRPTSSGDLYYIGT	461–494	Outside	0.7251
	495–517	TM helix
	518–546	Inside

(b)

G protein	Position of the selected sequences	Prediction of transmembrane helix using TMHMM server	VaxiJen value
PSKVIKSYYGTMDIKKINEGLLDSKILSAFNTVIALLGSIVIIVMNIMIIQN; YTRSTDNQA	21–47	Outside	0.38
	48–67	TM helix
	68–81	Inside
IQQQIKGLADKIGTEIGPKVSLIDTSSTITIPANIGLLGSKISQSTASINENV; NEKCKFTLPPLKIHECNISCPNPLPFREY	89–171	Outside	0.62
PQTEGVSNLVGLP	173–185	Outside	0.2138
NICLQKTSNQILKPKLISYTLPVVGQSGTCITDPLLAMDEGYFAYSHLE	187–235	Inside	0.58
IGSCSRGVSKQRIIGVGEVLDRGDEVPSLFMTNVW	237–271	Inside	0.50
NPNTVYHCSAVYNNEFYYVLCAVS	275–298	Inside	0.28
LNSTYWSGSLMMTRLAVKPK	305–315	Inside	0.78
YDKVMPYGPSGIKQGDTLYFPAVGFLVRTEF	345–375	Outside	0.84
CQYSKPENCRLSMGIRPNSHYILRSGLLKYNLSD	387–421	Inside	0.27
YDSLGQPVFYQASFSWDTMIKFGDV	445–469	Outside	0.61
CWEGVYNDAFLIDRINWISAGVFLDSNQTAENPVFTVFKDNE	503–544	Outside	0.33
LYRAQLASEDTNAQKTITNCFLLKNKIWCISLVEIYDTGDNVIRPKLFA; VKIPEQCT	546–602	Outside	0.59
VFIEISDQRLSIGSPSK	427–443	Outside	1.1465
TVNPLVVNWR	471–480	Inside	1.6557
NTVISRPGQSQCPRFN	482–497	Inside	−0.0237