Prediction of B-Cell Epitopes in Listeriolysin O, a Cholesterol Dependent Cytolysin Secreted by Listeria monocytogenes

Figure 1

ClustalW alignment of antigenic LLO amino acid sequences from GenBank. Red Letters denote the PEST sequence. Amino acids highlighted in red belong to domain 1, amino acids highlighted in blue belong to domain 2, amino acids highlighted in yellow belong to domain 3, and amino acids highlighted in green belong to domain 4. Amino acids highlighted in magenta are polymorphic. Black triangle denotes the location of signal sequence cleavage site. Purple amino acids denote the location of the undecapeptide sequence, required for the formation of the oligomeric pore complex. The purple α and brown β symbols above the alignments denote the locations of alpha helices and beta-pleated sheets, respectively, predicted by the Porter Secondary Structure Prediction method.