In Silico Analysis of Gene Expression Network Components Underlying Pigmentation Phenotypes in the Python Identified Evolutionarily Conserved Clusters of Transcription Factor Binding Sites

Figure 6

Orthologous multiple sequence alignment of LEF-1 across species. Note regions of divergence as well as some areas of strong conservation. Specifically, the N-terminal region exhibits some variability in amino acids exemplified by variable length repeats of the amino acid glycine near the beginning of the sequences. Stronger patterns of conservation are observable beginning at alignment position 146 and continuing at alignment position 220 which partly overlaps with the CTNNB1 binding, N-terminal domain (position 1 to position 209 in the python sequence). The DNA binding region of LEF-1 exhibits identical amino acid composition among all the species except for the king cobra (for which the sequence is annotated as partial).