Research Article
Bioinformatics Approach for Prediction of Functional Coding/Noncoding Simple Polymorphisms (SNPs/Indels) in Human BRAF Gene
Table 5
Predicted results of I-Mutant software.
| | Protein ID | Amino acid position | WT | MT | PH | Temperature (°C) | SVM2 prediction effect | DDG value prediction kcal/mol | RI |
| | ENSP00000288602 | 466 | G | R | 7.0 | 25 | Increase | | 4 | | ENSP00000288602 | 469 | G | R | 7.0 | 25 | Increase | | 4 | | ENSP00000288602 | 466 | G | V | 7.0 | 25 | Increase | | 2 | | ENSP00000288602 | 466 | G | E | 7.0 | 25 | Decrease | | 6 | | ENSP00000288602 | 469 | G | E | 7.0 | 25 | Increase | | 5 | | ENSP00000288602 | 595 | F | L | 7.0 | 25 | Decrease | | 8 | | ENSP00000288602 | 596 | G | R | 7.0 | 25 | Decrease | | 1 | | ENSP00000288602 | 596 | G | V | 7.0 | 25 | Decrease | | 2 | | ENSP00000288602 | 595 | F | S | 7.0 | 25 | Decrease | | 8 | | ENSP00000288602 | 638 | D | E | 7.0 | 25 | Decrease | | 7 | | ENSP00000418033 | 24 | G | R | 7.0 | 25 | Decrease | | 8 | | ENSP00000418033 | 66 | D | E | 7.0 | 25 | Increase | | 3 | | ENSP00000419060 | 109 | E | K | 7.0 | 25 | Decrease | | 8 | | ENSP00000419060 | 203 | F | S | 7.0 | 25 | Decrease | | 8 | | ENSP00000419060 | 246 | D | E | 7.0 | 25 | Decrease | | 7 |
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WT: wild type amino acid. MT: mutant type amino acid. DDG: delta DG (units of free energy) (DDG < 0: decreased stability, DDG > 0: increased stability). RI: reliability index.
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