Mix and Inject: Reaction Initiation by Diffusion for Time-Resolved Macromolecular Crystallography

Table 1

Diffusion times 𝜏_𝐷 for various crystal sizes from calculation, simulation, and experiment.

Crystal size	𝜏𝐷
400 × 400 × 1600 𝜇m^3	16 s (3)
	24 s [60]
	<1 min [59]
300 × 400 × 500 𝜇m^3	9.5 s
10 × 20 × 30 𝜇m^3	15 ms
3 × 4 × 5 𝜇m^3	1 ms
^a1 × 2 × 3 𝜇m^3	150 𝜇s
0.5 × 0.5 × 0.5 𝜇m^3	17 𝜇s
^b0.1 × 0.2 × 0.3 𝜇m^3	1.5 𝜇s

aWith much smaller crystals mixing times might be slower than diffusion times.
^bS. oneidensis ccNIR crystals would have about 4000 unit cells.