An Efficient Protocol for Deriving Liver Stem Cells from Neonatal Mice: Validating Its Differentiation Potential

Figure 3

Immunophenotyping of LDSCs. Passaged 2 LDSCs were stained with fluorescein isothiocyanate (FITC) conjugated anti-mouse EpCAM, CD44, GATA-4, CD105, CD90, CD34, CK18 (Cytokeratin 18 (CK)), CD29, Sca-1, and CD45 along with its corresponding isotype control IgG1. FACS results showed that these cells were strong positive for liver stem cell markers EpCAM, GATA-4, CD44, CD105, CK18, and CD29 (β₁ integrin) in addition to weak positive for Sca-1 and negative for hematopoietic markers CD45, CD34, and CD 90.