Research Article
An Efficient Protocol for Deriving Liver Stem Cells from Neonatal Mice: Validating Its Differentiation Potential
Table 2
Summary of the phenotype and genotype of isolated LDSCs.
| FACS |
| Liver marker | Expression |
| EpCAM | +++ | GATA-4 | +++ | CK18 | +++ |
| Mesenchyme marker | |
| CD105 | +++ | CD29 | +++ | CD44 | +++ | Sca-1 | ++ |
| Hematopoietic marker | |
| CD34 | − | CD45 | − | CD90 | − |
| RT-PCR |
| Transcriptional markers | Expression |
| HNF-3β | ++ | HNF-6 | + | C/EBP-α | ++ | C/EBP-β | ++ | HNF-1α | − | HNF-1β | − | HNF-3α | − | GATA-4 | ++ |
| Structural markers | |
|
CK8 | − | CK18 | ++++ | CK19 | + |
| Functional markers | |
| Albumin | − | Transthyretin | − | α-fetoprotein | − | AAT | − | p450 Cyp3a11 | ++ | PXMP1-L | + |
|
|
Percentage of expression: ++++: 95–100%, +++: 75–95%, ++: 40–75%, +: 10–35%, and −: 0-1%.
|