An Efficient Protocol for Deriving Liver Stem Cells from Neonatal Mice: Validating Its Differentiation Potential

Table 2

Summary of the phenotype and genotype of isolated LDSCs.

FACS
Liver marker	Expression
EpCAM	+++
GATA-4	+++
CK18	+++
Mesenchyme marker
CD105	+++
CD29	+++
CD44	+++
Sca-1	++
Hematopoietic marker
CD34	−
CD45	−
CD90	−
RT-PCR
Transcriptional markers	Expression
HNF-3β	++
HNF-6	+
C/EBP-α	++
C/EBP-β	++
HNF-1α	−
HNF-1β	−
HNF-3α	−
GATA-4	++
Structural markers
CK8	−
CK18	++++
CK19	+
Functional markers
Albumin	−
Transthyretin	−
α-fetoprotein	−
AAT	−
p450 Cyp3a11	++
PXMP1-L	+

Percentage of expression: ++++: 95–100%, +++: 75–95%, ++: 40–75%, +: 10–35%, and −: 0-1%.