Role of the IL-6 Gene in the Etiopathogenesis of Idiopathic Scoliosis

Table 2

PCR-RFLP protocol.

Gene, polymorphism	Annealing temperature, °C	PCR product size, bp	Restriction enzyme	Restriction fragments, bp
IL-6; rs1800795	58	198	SfaNI	CC: 198; CG: 198 + 140 + 58; GG: 140 + 58

PCR indicates polymerase chain reaction; RFLP: restriction fragment length polymorphism; IL-6: interleukin-6; bp: base pair.