Aloesin Suppresses Cell Growth and Metastasis in Ovarian Cancer SKOV3 Cells through the Inhibition of the MAPK Signaling Pathway

Figure 2

Aloesin arrests cell cycle and promotes cell apoptosis in SKOV3 cells. (a) SKOV3 cells without or with different doses of aloesin treatments were subject to cell cycle analysis by flow cytometry. Horizontal ordinate: channels (PE-A). Ordinate: cell number. (b) Western blot analysis of cell cycle-related proteins in both cell lines. β-actin was used as a loading control. Data represent the mean ± SD of three independent experiments. (c) Changes in apoptotic nuclear morphology were observed by Hoechst 33342 staining and visualized by fluorescent microscopy. (d) Cells were analyzed by flow cytometry with Annexin V-FITC/PI staining after aloesin treatment. Annexin V versus PI plots from the gated cells showed the populations corresponding to viable (Annexin V−/PI−) and necrotic (Annexin V−/PI+), early (Annexin V+/PI−), and late (Annexin V+/PI+) apoptotic cells. Horizontal ordinate: Annexin V-FITC. Ordinate: PI-PE. (e) Western blot analysis of apoptosis-related proteins in both cell lines. β-actin was used as a loading control. Data represent the mean ± SD of three independent experiments. , versus control.

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