Molecular Detection of EMT Markers in Circulating Tumor Cells from Metastatic Non-Small Cell Lung Cancer Patients: Potential Role in Clinical Practice

Figure 3

Recovery experiments of spiked A549^EMT cells and linearity assessment of control methods for cell count. (a) qIF and CF cell counts of spiked A549^EMT cells. All dilutions were analyzed in parallel; the mean recovery rates at lower dilutions (10¹ and 10²) were of 61 ± 14% and 80 ± 17% for qIF and 84 ± 16% and 56 ± 32% for CF. (b) Evaluation of the linearity of qIF and CF cell counts. The linearity of both methods was verified by linear analysis of regression curve (r²: 0.88 and 0.97 for qIF and CF assays, resp.).

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(b)