Molecular Detection of EMT Markers in Circulating Tumor Cells from Metastatic Non-Small Cell Lung Cancer Patients: Potential Role in Clinical Practice

Table 1

Primers used for target and housekeeping genes.

GAPDH	For 5-CATCAGCAATGCCTCCTGCAC-3
GAPDH	Rev 5-GTCATGAGTCCTTCCACGATACCAA-3′
CEA	For 5-AGGACAGAGCAGACAGCAGAG-3
CEA	Rev 5-GGTTCCAGAAGGTTAGAAGTGAGG-3
CK19	For 5-CCTGACACCATTCCTCCCTTC-3
CK19	Rev 5-CCGACGACTGGCGATAGC-3
ZEB1	For 5-GGGAGGAGCAGTGAAAGAGA-3
ZEB1	Rev 5-TTTCTTGCCCTTCCTTTCTG-3
ZEB2	For 5-AAGCCAGGGACAGATCAGC-3
ZEB2	Rev 5-CCACACT CTGTGCATTTGAACT-3
Snail1	For 5-GCTGCAGGACTCTAATCCAGA-3
Snail1	Rev 5-ATCTCCGGAGGTGGGATG-3
Snail2	For 5-TGGTTGCTTCAAGGACACAT-3
Snail2	Rev 5-GCAAATGCTCTGTTGCAGTG-3
Twist1	For 5-AGCTACGCCTTCTGGTCT-3
Twist1	Rev 5-CCTTCTCTGGAAACAATGACATC-3
Twist2	For 5-CATGTCCGCCTCCCACTA-3
Twist2	Rev 5-GCATCATTCAGAATCTCCTCCT-3
Vimentin	For 5-AATCCAAGTTTGCTGACCTCTCTG-3
Vimentin	Rev 5-TCATTGGTTCCTTTAAGGGCATCC-3