Immunological Alterations due to Hemodialysis Might Interfere with Early Complications in Renal Transplantation
Table 2
Leucocyte subsets before, during, and after hemodialysis.
Before dialysis
After one hour of dialysis
At the end of dialysis
15 minutes after end of dialysis
Reference range
Natural killer cells (% of lymphocytes)
Median (min–max)
9.1 (3.7-22.1)
7.3 (3-19.8)
11.5 (3.8-19)
11.6 (3.4-21.6)
3-22
Natural killer T cells (% of lymphocytes)
Median (min–max)
1.9 (0.4-8.6)
1.6 (0.1-5.1)
1.9 (0.4-5.9)
2.1 (0.3-7.1)
2.1-13.7
CD4+ T cells (% of lymphocytes)
Median (min–max)
50.9 (36.8-66.5)
53.8 (39.5-68.8)
52.3 (43.2-64.3)
51.7 (42.6-64.5)
31-51
CD8+ T cells (% of lymphocytes)
Median (min–max)
24.1 (13.6-41.4)
22.5 (13.5-27.2)
20.8 (11.6-33.8)
22.2 (11.8-31)
18-35
CD4- CD8- T cells (% of T cells)
Median (min–max)
4.2 (1.9-7.7)
3.7 (1.6-6.3)
3.3 (1.5-5.4)
3.4 (1.7-6.5)
3-10.2
CD25+ T cells (% of T cells)
Median (min–max)
37.4 (24.4-55)
38.6 (26.9-54.8)
40.6 (27.4-59.1)
39.5 (26.8-56.4)
22.9-44.9
CD4+ HLA-DR+ T cells (% of CD4+ T cells)
Median (min–max)
9.1 (2.8-21.7)
9.4 (2.8-21.1)
8.5 (3.3-27.7)
8.3 (3.4-28.4)
5-25
CD8+ HLA-DR+ T cells (% of CD8+ T cells)
Median (min–max)
23.5 (1.8-55.4)
18.6 (1.6-53.3)
18 (1.8-59.7)
21.9 (1.4-61.4)
5-25
Naive CD8+ T cells (% of CD8+ T cells)
Median (min–max)
16.5 (8.5-43.7)
22.1 (11-53.2)
16.6 (6.8-46.4)
18.7 (6.8-56.5)
28.4-66.7
CD8+ effector cells (% of CD8+ T cells)
Median (min–max)
31.2 (15.2-75.3)
28.1 (3.7-52.1)
29.7 (8.6-62.5)
30.9 (10.1-59.3)
7.4-24.6
CD8+ CCR5+ effector cells (% of CD8+ T cells)
Median (min–max)
9.3 (0.2-29.9)
6 (0.7-26)
10.4 (1.2-25.6)
10.5 (1-31.5)
< 5.9
Regulatory T cells (% of T cells)
Median (min–max)
6.2 (4.3-9.2)
6.9 (4.7-10.1)
6.9 (4.6-10)
7.2 (4.5-10)
2.8-7.2
HLA-DR+ Tregs (% of Tregs)
Median (min–max)
25.1 (9.3-38.4)
28.2 (9.5-38.5)
25.7 (8.6-45.8)
26.6 (9.4-46)
5.9-18.8
Eosinophils (% of leukocytes)
Median (min–max)
4 (1-10)
3 (0-7)
3 (0-6)
2 (0-6)
1-3
Neutrophils (% of leukocytes)
Median (min–max)
63 (51-77)
68 (46-87)
68 (52-79)
67 (50-87)
55-75
Median (min (minimum)–max (maximum)) in percent in fluorescence-activated cell sorting analysis (FACS) except for neutrophil and eosinophil counts (differential blood count by microscopy). Statistical analysis with nonparametric tests are shown in Table 3.
