Cytochrome P450 Epoxygenase-Dependent Activation of TRPV4 Channel Participates in Enhanced Serotonin-Induced Pulmonary Vasoconstriction in Chronic Hypoxic Pulmonary Hypertension

Figure 2

The effect of HC-067047 and MS-PPOH in WT and trpv4^-/- mice in chronic hypoxia. (a) Cumulative concentration-response curve of 5-HT in PAs of WT mice and trpv4^-/- mice with MS-PPOH preincubation and HC-067047 preincubation. Data are expressed as a percentage of K⁺-induced contractile responses (60 mM). (b, c) Bar graph showing the contractile response of hypoxic PAs of WT mice and trpv4^-/- mice to serotonin with HC-067047 or MS-PPOH preincubation. Mean values of maximal response () and is derived from experiments shown in (a). and were calculated from each individual PA using the 3-parameter logistic equation described in Materials and Methods. (d) Bar graph showing percent inhibition of K⁺-induced contraction by HC-067047 and MS-PPOH in WT mice. ; ns: nonsignificant.

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