Antigen-Experienced CD4lo T Cells Are Linked to Deficient Contraction of the Immune Response in Autoimmune Diabetes
Figure 3
“CD4lo” T cells are increased among NOD Con A blasts. (a) Spleen cells from naïve NOD or BALB/c mice were loaded with CFSE and stimulated for 72 hrs with Con A. The resulting blasts were stained with anti-CD4-PE and analyzed by flow cytometry. The results are representative of four experiments. The data are expressed as dot plots of ungated cells (100,000 events). The numbers in the plots represent the percentage of total cells in regions R1 (CD4 MFI 800) and R2 (CD4 MFI 250). (b) The values in the bar graph reflect the mean percentage of cells in each category, from 3 separate experiments (100,000 events collected for each sample). Student’s -test was used to compare responses between NOD and BALB/c blasts (only -values reaching significance, .05, are shown).
