Antigen-Experienced CD4lo T Cells Are Linked to Deficient Contraction of the Immune Response in Autoimmune Diabetes

Table 2

The addition of cytokines during primary stimulation increases the transition of naïve NOD T cells to CD4hi effectors.

Primary stimulationa	24 hrb		72 hrb
	CD4loc	CD4hi	CD4lo	CD4hi
Con A	58%	10	50	12
Con A+IL-2	42∗	14	25∗	21∗
Con A+IL-4	42∗	25∗	14∗	39∗
Con A+IL-7	42∗	15	22∗	28∗
Con A+IL-15	40∗	30∗	19∗	40∗

a Splenocytes were cultured in 24-well plates for 72 hrs with 2.0 𝜇g/mL of Con A, with or without recombinant mouse IL-2, IL-4, IL-7, or IL-15.
b Twenty-four or 72 hrs after the addition of Con A, the cells were collected and stained with anti-CD4-PE and analyzed by flow cytometry. The data are expressed as mean percentage of “CD4lo” and “CD4hi” cells in triplicate wells, amongst the total CD4+ population. The SD was less than 20%. The asterisk (∗) indicates that 𝑃<.05 when compared to Con A blasts induced without cytokines added. The results are representative of two experiments.