A Comparison of Epitope Repertoires Associated with Myasthenia Gravis in Humans and Nonhuman Hosts
Table 1
(a) MG-related epitopes derived from AChR of different species. (b) MG-related epitopes derived from antigen other than AChR.
(a)
Antigen
Positive
Negative
Total tested
References
Acetylcholine receptor (AChR)
Human epitopes
338
80
418
90
T-cell assays
704
575
1279
B cell assays
194
71
265
T. californica epitopes
175
60
235
102
T-cell assays
495
426
921
B cell assays
462
53
515
Chicken epitopes
21
16
37
3
T-cell assays
0
0
0
B cell assays
26
16
42
Rat epitopes
16
0
16
6
T-cell assays
19
0
19
B cell assays
44
0
44
Bovine epitopes
11
0
11
3
T-cell assays
11
6
17
B cell assays
5
0
5
T. marmorata epitopes
6
0
6
3
T-cell assays
6
0
6
B cell assays
5
0
5
Mouse epitopes
5
3
8
8
T-cell assays
7
4
11
B cell assays
12
0
12
Data was derived from the IEDB Search interface using the Epitope Source Molecule Finder to retrieve epitopes from all AChRs subunits in B cell and T-cell response assays, irrespective of the host in which the immune response was described. Search criteria included “acetylcholine receptor” or “cholinergic receptor” (in the case of rats) for the Molecule Name and the AChR species from the Organism Finder (e.g., Mus musculus, human, Torpedo, etc.). In cases where multiple AChRs accession numbers were linked to the various references, all available identifiers were used, including protein tree identifiers (ex. PROTREE (PT10006210)) and Internal identifiers (ex. AChR IEDB (SRC248684)). Listed in the table are the total numbers of epitopes (distinct molecular structure) and then distinct assays captured within the database. By definition this number does not indicate the number of individual epitopes, as each epitope is defined experimentally by one or more assays.
(b)
Antigen
Epitopes
Negative peptides
Total tested
References
β-2 adrenergic receptor
1
Human
3
0
3
T-cell assays
0
0
0
B cell assays
5
0
5
Search criteria included “adrenergic receptor” for the Molecule Name, and each different species for which data was available (as determined from the list of hosts in the keyword search) from the Organism Finder. In the case of adrenergic receptor, though a total of 7 references were found, only 1 (PMID: 1378277) was specific to MG epitopes; the others were related to other autoimmune disease states. Listed in the table are the total numbers of epitopes (distinct molecular structure) and then distinct assays captured within the database. By definition this number does not indicate the number of individual epitopes, as each epitope is defined experimentally by one or more assays.
