A Novel Method for Real-Time, Continuous, Fluorescence-Based Analysis of Anti-DNA Abzyme Activity in Systemic Lupus
Figure 3
Detection of hydrolysis of ss herring sperm DNA by Agilent 2100 Bioanalyzer (DNA7500 microchip) digital analysis. *Agilent 2100 Lab-on-Chip digital analysis comparison of hydrolysis of ss herring sperm DNA (obtained by heating and fast cooling) by DNAse I control and anti-poly-(dT) ssDNA antibody sample purified from lupus patient serum demonstrates that lupus anti-poly-(dT) ssDNA antibody is hydrolytic. (a) Lane 1: ss herring sperm DNA with DNAse I at T = 0. Lane 2: ss herring sperm DNA, no DNAse I. Lane 3: ss herring sperm DNA with DNAse I at T = 5 min. Lane 4: lambda phage, no DNAse I. Lanes 5, 6: ss herring sperm DNA with DNAse I at T = 30 min. Lane 7: lambda phage with DNAse I at T = 30 min. Lanes 8: lambda phage, no DNAse I. Lanes 9, 10: same as 5, 6. Lanes 11, 12: DNAse I, no substrate. (b) Lanes 1–4: ss herring sperm DNA with lupus anti-ssDNA antibody at T = 3 hrs. Lanes 5–8: lambda phage DNA, no antibody.
