Posttranslational Protein Modification in the Salivary Glands of Sjögren’s Syndrome Patients
Figure 1
(a) Chromatogram shows the purification of anti-CCP conjugated antibody that was further fractionated using gel filtration chromatography, which produced three fractions. The first pool (tubes 9–11) contained the HRP-anti-CCP conjugates (green) second pool (tubes 12–14) (pink). Pool 3 (tubes 18–20) contained the nonconjugated peroxidase fraction (blue). (b) ELISA graph of different fractions obtained by gel filtration. Pool 1 was very reactive for CCP, with an optical density that was threefold higher than the original serum titre. Pool 2 activity was weaker, and pool 3 was negative. (OD: optical density).
