A Comparison of Anti-Nuclear Antibody Quantification Using Automated Enzyme Immunoassays and Immunofluorescence Assays

Table 3

Comparison of immunofluorescence patterns and titers between HEp-2000 immunofluorescence (Immuno Concept) and combined ANA immunoassays (Phadia). The combined EIA results from dsDNA, ANA Symphony, and the 7 subantigens (SmD, U1RNP, SSA/Ro, SSB/La, Scl-70, CENP-B, and Jo-1) are shown.

EIA-combined	NEG	POS^?	CEN^?	CEN	HOM^+	HOM	NUC^?	NUC and HOM	NUC	SPE^?	SPE^+	SPE	MIT
NEG	308	1			12	5	1	1	6	1	2	3	1
CENP-B				2
dsDNA	7	1	1			2
dsDNA	4					1
dsDNA, SSA/Ro	1
dsDNA, SSA/Ro	1	1
dsDNA, La, SSA/Ro												1
dsDNA, RnpU1, SmD, SSA/Ro						1
SSB/La	1
SSB/La, SSA/Ro	1											1
SSB/La, SSA/Ro, CENP-B				1
RnpU1	2								1			2
RnpU1	2
RnpU1, SSA/Ro												1
RnpU1, SmD												1
SSA/Ro	4	1
SSA/Ro, CENP-B	1
Scl-70						1
SmD	1

Primary laboratory: Gentofte Hospital; NEG: negative; POS: positive; CEN: centromere; HOM: homogeneous; NUC: nuclear; SPE: speckled; MIT: mitochondria; ?: uncertain reaction; +: weak reaction; ++: intermediate reaction, and +++: strong reaction. Underlining and bold font indicate agreement, italics indicate acceptable agreement, and double underlining indicates disagreement.