Research Article
A Comparison of Anti-Nuclear Antibody Quantification Using Automated Enzyme Immunoassays and Immunofluorescence Assays
Table 4
Comparison between combined ANA immunoassay (Phadia) and HEp-2000 cell immunofluorescence (Immuno Concept). The combined results of dsDNA, ANA Symphony, and the 7 subantigens constituted the EIA-combined assay, and the combined results of IFA using HEp-2000 cells from two laboratories constituted the HEp-2000-combined assay. The results of the immunoassays were considered equivocal if the anti-dsDNA levels were between 10–15 IU/mL or if the Symphony results were between 0.7–1.0; however, the 7 subantigen results overruled those of the Symphony. The HEp-2000 results were considered equivocal if the two laboratories’ results were discordant. The degree of agreement was fair (Kappa statistic = 0.29).
| |||||||||||||||||||||||||||||||||||||||||||||||||