Exploring T Cell Reactivity to Gliadin in Young Children with Newly Diagnosed Celiac Disease

Figure 2

Recombinant peptide/MHC protein stimulates IFN-𝛾 production from bulk unfractionated PBMCs. (a) Stimulation of PBMCs from a single subject in triplicate having both HLA-DQ8 and DQ2 alleles showing response to the recombinant proteins greater than background. The IFN-𝛾 response is DQ restricted as it can be blocked with a monoclonal DQ antibody. An anti-CD3 monoclonal antibody is used to stimulate T cells as a positive control. The 𝛼-gliadin p1E, p9E peptide (QQYPSGEGSFQPSQENPQ) is present in the DQ8 recombinant protein, while 𝛼-I gliadin (QLQPFPQPELPY) is present in the DQ2 protein. (b) Summative stimulation data from nine subjects all with HLA-DQ2 (DQA∗05:01, DQB∗02:01). (c) Data from three subjects having the HLA-DQ8 (DQA∗03:01, DQB∗03:02) allele.

(a)

(b)

(c)